Linked Life Data

14632210

Source:http://linkedlifedata.com/resource/pubmed/id/14632210

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2003-11-24
pubmed:abstractText
Fifty-one mycosis fungoides samples were analyzed for microsatellite instability (MSI) using the panel of markers recommended for hereditary nonpolyposis colorectal cancer kindred and a panel we designed for cutaneous T cell lymphoma in order to compare detection rates and determine if MSI is a genome-wide phenomenon. Samples demonstrating MSI were analyzed for abnormalities of the hMLH1 gene including loss of heterozygosity, mutations, and promoter hypermethylation. MSI was detected in 16% using the hereditary nonpolyposis colorectal cancer panel and 22% with the cutaneous T cell lymphoma panel. Overall, 27% demonstrated MSI and 73% had a stable phenotype. hMLH1 gene studies did not detect loss of heterozygosity or reveal any mutations. Promoter hypermethylation was detected in nine of 14 patients with MSI, however (64%). In addition hMLH1 and hMSH2 protein expression was studied using immunohistochemical techniques. Five of nine patients with MSI and hMLH1 promoter methylation showed abnormal hMLH1 protein expression with normal hMSH2 gene expression. All other patients tested demonstrated normal hMLH1 and hMSH2 protein expression. MSI was found to be more prevalent in tumor stage mycosis fungoides (47%) than early stage disease (20%) and was associated with an older age of onset of mycosis fungoides. MSI may be a consequence of hMLH1 promoter hypermethylation in mycosis fungoides patients and may prevent transcription in a subset of patients. This suggests that the development of a mutator phenotype may contribute to disease progression in mycosis fungoides.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0022-202X
pubmed:author
pubmed:issnType
Print
pubmed:volume
121
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
894-901
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:14632210-Adaptor Proteins, Signal Transducing, pubmed-meshheading:14632210-Carrier Proteins, pubmed-meshheading:14632210-DNA Methylation, pubmed-meshheading:14632210-DNA-Binding Proteins, pubmed-meshheading:14632210-Female, pubmed-meshheading:14632210-Gene Expression, pubmed-meshheading:14632210-Humans, pubmed-meshheading:14632210-Immunohistochemistry, pubmed-meshheading:14632210-Loss of Heterozygosity, pubmed-meshheading:14632210-Male, pubmed-meshheading:14632210-Microsatellite Repeats, pubmed-meshheading:14632210-Middle Aged, pubmed-meshheading:14632210-MutS Homolog 2 Protein, pubmed-meshheading:14632210-Mycosis Fungoides, pubmed-meshheading:14632210-Neoplasm Proteins, pubmed-meshheading:14632210-Nuclear Proteins, pubmed-meshheading:14632210-Phenotype, pubmed-meshheading:14632210-Promoter Regions, Genetic, pubmed-meshheading:14632210-Proto-Oncogene Proteins, pubmed-meshheading:14632210-Skin Neoplasms, pubmed-meshheading:14632210-Survival Rate
pubmed:year
2003
pubmed:articleTitle
Microsatellite instability is associated with hypermethylation of the hMLH1 gene and reduced gene expression in mycosis fungoides.
pubmed:affiliation
Skin Tumour Unit, St John's Institute Dermatology, St Thomas' Hospital, London, UK. juliascarisbrick@doctors.org.uk
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't