Source:http://linkedlifedata.com/resource/pubmed/id/14632194
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
2003-11-24
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pubmed:abstractText |
Previously, we reported the identification of autoantibodies against the melanin-concentrating hormone receptor 1 in patients with vitiligo. In this study, the B cell epitopes on melanin-concentrating hormone receptor 1 that are recognized by these autoantibodies have been identified. Deletion derivatives of melanin-concentrating hormone receptor 1 complementary DNA were constructed and then translated in vitro with the concomitant incorporation of [35S]-methionine into the protein products. The [35S]-labeled melanin-concentrating hormone receptor 1 derivatives were subsequently used in radio-binding assays to investigate the reactivity of sera from nine vitiligo patients that were known to contain antibodies to the receptor. Analysis of the results obtained in the radio-binding assays suggested the existence of multiple antibody binding sites on melanin-concentrating hormone receptor 1, including regions between amino acids 1 to 138 and 139 to 298. Several patients exhibited autoantibodies to more than one melanin-concentrating hormone receptor 1 epitope indicating a heterogeneous humoral response to the receptor. Computer prediction of the potential B cell epitopes on melanin-concentrating hormone receptor 1 revealed that the epitope domains identified overlapped, at least in part, with regions predicted to be highly antigenic.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Autoantibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Autoantigens,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes, B-Lymphocyte,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Pituitary Hormone,
http://linkedlifedata.com/resource/pubmed/chemical/melanin-concentrating hormone...
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0022-202X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
121
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
765-70
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:14632194-Adult,
pubmed-meshheading:14632194-Aged,
pubmed-meshheading:14632194-Antibody Specificity,
pubmed-meshheading:14632194-Autoantibodies,
pubmed-meshheading:14632194-Autoantigens,
pubmed-meshheading:14632194-DNA, Complementary,
pubmed-meshheading:14632194-Epitopes, B-Lymphocyte,
pubmed-meshheading:14632194-Female,
pubmed-meshheading:14632194-Gene Deletion,
pubmed-meshheading:14632194-Humans,
pubmed-meshheading:14632194-Male,
pubmed-meshheading:14632194-Middle Aged,
pubmed-meshheading:14632194-Radioimmunoassay,
pubmed-meshheading:14632194-Receptors, Pituitary Hormone,
pubmed-meshheading:14632194-Vitiligo
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pubmed:year |
2003
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pubmed:articleTitle |
Autoantibodies in vitiligo patients recognize multiple domains of the melanin-concentrating hormone receptor.
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pubmed:affiliation |
Division of Clinical Sciences (North), University of Sheffield, Sheffield, UK.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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