Linked Life Data

14632082

Source:http://linkedlifedata.com/resource/pubmed/id/14632082

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
17
pubmed:dateCreated
2003-11-24
pubmed:abstractText
We report on a simple methodology to move selected single live cells to a desired location on a flat substrate, such as a patterned biomicroelectromechanical system chip. A macroscopic syringe-and-tube-based hydrodynamic manipulation system is used to achieve controlled cell navigation onto hydrophilic sites for cell attachment. Centimeter-per-second flow velocities generated by the system get downgraded to micrometers-per-second flow at the height of settled cells as a result of viscous flow in the medium. By pushing/pulling two syringes that produce two orthogonal flows, fine manipulation in any horizontal direction is feasible. After attachment of the desired cell(s) onto the selected hydrophilic site, all other unwanted cells are washed away from the surrounding hydrophobic surface with faster flow. This simple methodology is applicable for rapid cell pattern formation with high precision.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0003-2700
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
75
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4686-90
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Hydrodynamic micromanipulation of individual cells onto patterned attachment sites on biomicroelectromechanical system chips.
pubmed:affiliation
Department of Biomedical Engineering, Case Western Reserve University, Cleveland, Ohio 44106, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't