Source:http://linkedlifedata.com/resource/pubmed/id/14630789
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
7
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| pubmed:dateCreated |
2004-3-22
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| pubmed:abstractText |
The Runx1/core binding factor-beta (CBFbeta) transcriptional complex is required for the establishment of hematopoiesis during development. Despite its critical role during development, a detailed analysis of Runx1 expression within specific lineages and developmental stages of the adult hematopoietic system is lacking. To address this, we have developed a Runx1-green fluorescent protein (GFP) knock-in mouse. We show that Runx1 is expressed in several hematopoietic lineages, including myeloid, B-lymphoid, and T-lymphoid cells. By contrast, Runx1 is weakly expressed in early erythroid cells, and its expression is rapidly extinguished during later stages of erythropoiesis. Runx1 expression is induced during early B-cell development and is expressed at a uniform level during all subsequent stages of B-cell development. Within the thymus, Runx1 is expressed at the highest level in CD4-CD8- double-negative thymocytes. In peripheral T cells, Runx1 is differentially expressed, with CD4+ T cells expressing 2- to 3-fold higher levels of Runx1 than CD8+ cells. Taken together, these findings indicate that although widely expressed in the hematopoietic system, the expression of Runx1 is regulated in a cell type- and maturation stage-specific manner. In addition, the Runx1-IRES-GFP knock-in mouse strain should prove valuable for investigation of Runx1 function in adult hematopoiesis.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
AIM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Core Binding Factor Alpha 2 Subunit,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Green Fluorescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Luminescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RUNX1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Runx1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0006-4971
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
1
|
| pubmed:volume |
103
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
2522-9
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| pubmed:dateRevised |
2007-11-14
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| pubmed:meshHeading |
pubmed-meshheading:14630789-Adult,
pubmed-meshheading:14630789-Animals,
pubmed-meshheading:14630789-Base Sequence,
pubmed-meshheading:14630789-CD4-Positive T-Lymphocytes,
pubmed-meshheading:14630789-Core Binding Factor Alpha 2 Subunit,
pubmed-meshheading:14630789-DNA Primers,
pubmed-meshheading:14630789-DNA-Binding Proteins,
pubmed-meshheading:14630789-Gene Expression Regulation,
pubmed-meshheading:14630789-Genotype,
pubmed-meshheading:14630789-Green Fluorescent Proteins,
pubmed-meshheading:14630789-Hematopoiesis,
pubmed-meshheading:14630789-Humans,
pubmed-meshheading:14630789-Luminescent Proteins,
pubmed-meshheading:14630789-Lymph Nodes,
pubmed-meshheading:14630789-Lymphocyte Subsets,
pubmed-meshheading:14630789-Mice,
pubmed-meshheading:14630789-Mice, Transgenic,
pubmed-meshheading:14630789-Polymerase Chain Reaction,
pubmed-meshheading:14630789-Proto-Oncogene Proteins,
pubmed-meshheading:14630789-Transcription Factors
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| pubmed:year |
2004
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| pubmed:articleTitle |
Role of RUNX1 in adult hematopoiesis: analysis of RUNX1-IRES-GFP knock-in mice reveals differential lineage expression.
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| pubmed:affiliation |
Department of Pathology, St Jude Children's Research Hospital, Memphis, TN 38105, USA. robert.lorsbach@stjude.org
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|