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rdf:type |
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lifeskim:mentions |
umls-concept:C0017262,
umls-concept:C0025914,
umls-concept:C0026809,
umls-concept:C0086418,
umls-concept:C0205250,
umls-concept:C1171362,
umls-concept:C1334043,
umls-concept:C1424990,
umls-concept:C1428393,
umls-concept:C1515670,
umls-concept:C2323499
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pubmed:issue |
6
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pubmed:dateCreated |
2004-3-4
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pubmed:abstractText |
Comparison of the gene expression repertoire in human hematopoietic progenitors and mature leukocytes led to identification of a transcript expressed in CD34+cells and undetectable in differentiated cells. Sequencing of the cDNA (termed EHZF: early hematopoietic zinc finger) revealed 30 zinc fingers with 96% homology to mouse Evi3, a recently identified gene associated with the retroviral integration site in AKXD-27 B-cell lymphomas. EHZF and Evi3 share high homology with the transcription cofactor OAZ, implicated in the control of olfactory epithelium and B-lymphocyte differentiation and in the bone morphogenic protein (BMP) signal transduction. Here we show that (1) EHZF expression is abundant in human CD34+ progenitors and declines rapidly during cytokine-driven differentiation; (2) significant mRNA levels are found in most acute myelogenous leukemias; (3) in response to BMPs EHZF complexes SMADs 1 and 4, binds to, and enhances the transcriptional activity of, a BMP2/4 responsive element; (4) EHZF inhibits the transcriptional activity of early B-cell factor (EBF), a transcription factor essential for specification of the B-cell lineage. Taken together, our data suggest that EHZF is likely to play a relevant role in the control of human hematopoiesis and might be implicated in the development of hematopoietic malignancies.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Bone Morphogenetic Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/EBF1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Ebf1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Evi3 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Smad Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0006-4971
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pubmed:author |
pubmed-author:AgostiValterV,
pubmed-author:AmodioNicolaN,
pubmed-author:BondHeather MHM,
pubmed-author:BonelliPatriziaP,
pubmed-author:CarboneEnnioE,
pubmed-author:De RosaGennaroG,
pubmed-author:Di NicolaMassimoM,
pubmed-author:GianniAlessandro MAM,
pubmed-author:GriecoMicheleM,
pubmed-author:HataAkikoA,
pubmed-author:MesuracaMariaM,
pubmed-author:MooreMalcolm A SMA,
pubmed-author:MorroneGiovanniG,
pubmed-author:VenutaSalvatoreS
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
103
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2062-70
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:14630787-Amino Acid Sequence,
pubmed-meshheading:14630787-Animals,
pubmed-meshheading:14630787-Bone Morphogenetic Proteins,
pubmed-meshheading:14630787-Caco-2 Cells,
pubmed-meshheading:14630787-Carrier Proteins,
pubmed-meshheading:14630787-Cloning, Molecular,
pubmed-meshheading:14630787-DNA-Binding Proteins,
pubmed-meshheading:14630787-Gene Expression,
pubmed-meshheading:14630787-HL-60 Cells,
pubmed-meshheading:14630787-HeLa Cells,
pubmed-meshheading:14630787-Hematopoietic Stem Cells,
pubmed-meshheading:14630787-Humans,
pubmed-meshheading:14630787-Jurkat Cells,
pubmed-meshheading:14630787-Mice,
pubmed-meshheading:14630787-Molecular Sequence Data,
pubmed-meshheading:14630787-Nuclear Proteins,
pubmed-meshheading:14630787-RNA, Messenger,
pubmed-meshheading:14630787-Sequence Homology, Amino Acid,
pubmed-meshheading:14630787-Smad Proteins,
pubmed-meshheading:14630787-Trans-Activators,
pubmed-meshheading:14630787-Transcription, Genetic,
pubmed-meshheading:14630787-Transcription Factors,
pubmed-meshheading:14630787-U937 Cells,
pubmed-meshheading:14630787-Zinc Fingers
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pubmed:year |
2004
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pubmed:articleTitle |
Early hematopoietic zinc finger protein (EHZF), the human homolog to mouse Evi3, is highly expressed in primitive human hematopoietic cells.
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pubmed:affiliation |
Department of Experimental and Clinical Medicine Gaetano Salvatore, University of Catanzaro Magna, Graecia, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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