Linked Life Data

14625811

Source:http://linkedlifedata.com/resource/pubmed/id/14625811

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
33-34
pubmed:dateCreated
2003-11-19
pubmed:abstractText
From a posteriori analyses of genetic variation, recombination can only be identified when the parental genomes are distinct. For viruses like HIV-1, this requires the producer cell to be infected by more than one virus. Using fluorescence in situ hybridisation, the provirus copy numbers in splenocytes from two HIV-1 patients were determined. More than 75% of infected splenocytes harboured two or more proviruses, range 1-8, with a mean of approximately 3-4 per cell. Sequencing of amplified DNA from single laser micro-dissected cells showed an extraordinary degree of diversity while numerous recombinants were evident. Given the dynamics of HIV-1 turnover in vivo and a recombination rate of approximately 3 cross-overs per cycle, some genomes from a fifteen year old infection may have undergone as many cross-overs as bases in the genome. Thus, recombination profoundly influences HIV evolution and gives it a non-clonal and transitory nature in vivo.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1424-7860
pubmed:author
pubmed:issnType
Print
pubmed:day
23
pubmed:volume
133
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
451-4
pubmed:dateRevised
2011-2-15
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
The non-clonal and transitory nature of HIV in vivo.
pubmed:affiliation
Department of Virology, University of the Saarland, Homburg, Germany.
pubmed:publicationType
Journal Article, Review, Research Support, Non-U.S. Gov't