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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2003-11-19
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pubmed:abstractText |
This paper describes the methods that were used for the preparation of gp96-associated peptides and the analysis of these peptides using mass spectrometry. A general approach for stripping, enriching, and separating peptides associated with gp96 is presented. Protocols for the demonstration of the diversity of gp96-associated peptides using mass spectrometry and the identification of these peptides using a combination of tandem mass spectrometry and protein database searching are described. Important parameters and factors that affect the outcome of the experiments are discussed.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1046-2023
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
32
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
32-7
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pubmed:meshHeading |
pubmed-meshheading:14624875-Animals,
pubmed-meshheading:14624875-Antigens, Neoplasm,
pubmed-meshheading:14624875-Chromatography, High Pressure Liquid,
pubmed-meshheading:14624875-Heat-Shock Proteins,
pubmed-meshheading:14624875-Indicators and Reagents,
pubmed-meshheading:14624875-Mice,
pubmed-meshheading:14624875-Neoplasms,
pubmed-meshheading:14624875-Peptide Fragments,
pubmed-meshheading:14624875-Spectrometry, Mass, Electrospray Ionization,
pubmed-meshheading:14624875-Spectrometry, Mass, Matrix-Assisted Laser...
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pubmed:year |
2004
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pubmed:articleTitle |
Analytical challenges and strategies for the characterization of gp96-associated peptides.
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pubmed:affiliation |
Antigenics, Inc., Woburn, MA 01801, USA. cliu@antigenics.com
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pubmed:publicationType |
Journal Article
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