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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2003-11-18
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pubmed:abstractText |
Direct electron transfer of hemoglobin (Hb) can be achieved in a Hb/montmorillonite (MMT)/polyvinyl alcohol multi-assembly at a pyrolytic graphite (PG) electrode. Accordingly, a novel nitric oxide (NO) biosensor is proposed. The reduction of NO is observed at a potential of approximately -783 mV (vs. SCE) at pH 5.5. At optimum pH, this biosensor shows a wide linear range of 1.0x10(-6)-2.5x10(-4) mol/l with a detection limit of 5.0x10(-7) mol/l. The sensor-to-sensor reproducibility is good consideringmacr; a relative standard deviation of 3.5% in five independent determinations at 5.0x10(-5) mol/l NO. The modified electrode is conveniently constructed and durable in long-term operations.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0956-5663
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
30
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pubmed:volume |
19
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
441-5
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pubmed:dateRevised |
2009-7-14
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pubmed:meshHeading |
pubmed-meshheading:14623468-Bentonite,
pubmed-meshheading:14623468-Biosensing Techniques,
pubmed-meshheading:14623468-Coated Materials, Biocompatible,
pubmed-meshheading:14623468-Electrochemistry,
pubmed-meshheading:14623468-Electrodes,
pubmed-meshheading:14623468-Equipment Design,
pubmed-meshheading:14623468-Equipment Failure Analysis,
pubmed-meshheading:14623468-Graphite,
pubmed-meshheading:14623468-Hemoglobins,
pubmed-meshheading:14623468-Hot Temperature,
pubmed-meshheading:14623468-Manufactured Materials,
pubmed-meshheading:14623468-Nitric Oxide,
pubmed-meshheading:14623468-Polyvinyl Alcohol,
pubmed-meshheading:14623468-Reproducibility of Results,
pubmed-meshheading:14623468-Sensitivity and Specificity
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pubmed:year |
2003
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pubmed:articleTitle |
A nitric oxide biosensor based on the multi-assembly of hemoglobin/montmorillonite/polyvinyl alcohol at a pyrolytic graphite electrode.
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pubmed:affiliation |
Department of Biochemistry and National Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't,
Evaluation Studies,
Validation Studies
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