Linked Life Data

14623246

Source:http://linkedlifedata.com/resource/pubmed/id/14623246

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2003-11-18
pubmed:abstractText
The role of Src-family protein tyrosine kinases (SFKs) in egg activation has been established, in large part, by the observation that GST fusion proteins encoding the SH2 domain of Src or Fyn suppress the sperm-induced calcium transient and cause polyspermy in marine invertebrate eggs. These fusion proteins are thought to act as dominant-negative inhibitors of SFK function; however, the mechanism by which they work is not known. The objective of the present study was to test the hypothesis that fusion proteins containing the above SH2 domains prevent activation of SFKs in response to fertilization. A single cell assay was developed that allows estimation of SFK activity in eggs injected with the GST-Fyn-SH2 fusion protein. The results demonstrate that the GST-Fyn-SH2 fusion protein prevents fertilization induced stimulation of SFK activity at concentrations that also suppress the sperm-induced calcium transient in zebrafish eggs.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0012-1606
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
264
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
255-62
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Activation of Src-family PTK activity at fertilization: role of the SH2 domain.
pubmed:affiliation
Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, KS 66160, USA. wkinsey@kumc.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.