Linked Life Data

14622225

Source:http://linkedlifedata.com/resource/pubmed/id/14622225

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2003-11-18
pubmed:abstractText
Synucleins have attracted much attention because of their involvement in several neurodegenerative disorders. In a screening for genes differentially expressed after high-dose cocaine exposure, we found gamma-synuclein as a major upregulated candidate in the tegmentum. Overexpression of both alpha- and gamma-synuclein after drug treatment was confirmed by means of microarrays, yielding an increase in the hippocampus, the striatum and the tegmentum (2.65 x, 1.96 x and 3.5 x, respectively, for alpha-synuclein vs. 2.7 x, 1.96 x and 7.16 x for gamma-synuclein), but no change in the nucleus accumbens. Investigation of the distribution of mRNA (by in situ hybridization) and of the proteins (by immunocytochemistry) shows in both cases a clearly distinct pattern of expression for alpha- and gamma-synuclein. alpha-synuclein displays a very characteristic distribution, confined to specific nuclei, whereas gamma-synuclein is more widely expressed throughout the brain. mRNA of both alpha- and gamma-synucleins display a complementary pattern of expression all over the cortex. In contrast to gamma-synuclein, alpha-synuclein is neuronal, being only found in NeuN-expressing cells, and is expressed in the basal ganglia (faintly) and in the substantia nigra compacta where it is highly correlated with tyrosine hydroxylase. Immunocytochemistry shows that gamma-synuclein generally colocalizes with glial fibrillary acidic protein-expressing cells and is abundant in the red nucleus, the substantia nigra reticulata and the anterior commissure, while gamma-synuclein mRNA labels the matrix compartments of the caudate-putamen. The role of synucleins in relation to cocaine-induced plasticity or neurotoxicity is discussed.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0953-816X
pubmed:author
pubmed:issnType
Print
pubmed:volume
18
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1923-38
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:14622225-Anesthetics, Local, pubmed-meshheading:14622225-Animals, pubmed-meshheading:14622225-Brain, pubmed-meshheading:14622225-Brain Chemistry, pubmed-meshheading:14622225-Cell Count, pubmed-meshheading:14622225-Cocaine, pubmed-meshheading:14622225-Glial Fibrillary Acidic Protein, pubmed-meshheading:14622225-Immunohistochemistry, pubmed-meshheading:14622225-In Situ Hybridization, pubmed-meshheading:14622225-Male, pubmed-meshheading:14622225-Nerve Tissue Proteins, pubmed-meshheading:14622225-Oligonucleotide Array Sequence Analysis, pubmed-meshheading:14622225-Phosphopyruvate Hydratase, pubmed-meshheading:14622225-RNA, Messenger, pubmed-meshheading:14622225-Rats, pubmed-meshheading:14622225-Rats, Sprague-Dawley, pubmed-meshheading:14622225-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:14622225-Synucleins, pubmed-meshheading:14622225-Tissue Distribution, pubmed-meshheading:14622225-Tyrosine 3-Monooxygenase, pubmed-meshheading:14622225-alpha-Synuclein, pubmed-meshheading:14622225-gamma-Synuclein
pubmed:year
2003
pubmed:articleTitle
Distribution of alpha- and gamma-synucleins in the adult rat brain and their modification by high-dose cocaine treatment.
pubmed:affiliation
Max-Planck Institute of Psychiatry, München, Germany.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't