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rdf:type |
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lifeskim:mentions |
|
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pubmed:issue |
8
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pubmed:dateCreated |
2003-11-18
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pubmed:abstractText |
For neuroprotective therapy of neurodegenerative diseases creatine treatment has gained special interest because creatine has been shown to cross the blood-brain barrier, accumulate in the human brain in vivo and cause delayed neuronal cell death in a large number of animal models. Here, we used the pilocarpine model of temporal lobe epilepsy to determine whether creatine administration is able to attenuate the epilepsy-associated decrease in hippocampal N-acetyl aspartate (NAA) concentrations, impairment of mitochondrial function and neuronal cell loss. In vivo1H-NMR spectroscopy showed, in epileptic rats after creatine administration, higher hippocampal NAA concentrations, suggesting improved neuronal survival. However, in vitro observation of hippocampal slices from creatine-treated epileptic rats revealed a more pronounced loss of pyramidal neurons and decrease in activity of mitochondrial enzymes in hippocampal subfields. This indicates that NAA concentrations measured by in vivo1H-NMR spectroscopy reflect alterations of metabolism rather than neuronal cell densities. Our data indicate an adverse effect of creatine on neuronal survival under conditions of enhanced neuronal activity.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0953-816X
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:volume |
18
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2292-300
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:14622190-Animals,
pubmed-meshheading:14622190-Anticonvulsants,
pubmed-meshheading:14622190-Aspartic Acid,
pubmed-meshheading:14622190-Cell Count,
pubmed-meshheading:14622190-Creatine,
pubmed-meshheading:14622190-Diazepam,
pubmed-meshheading:14622190-Disease Models, Animal,
pubmed-meshheading:14622190-Epilepsy, Temporal Lobe,
pubmed-meshheading:14622190-Food, Formulated,
pubmed-meshheading:14622190-Hippocampus,
pubmed-meshheading:14622190-Magnetic Resonance Spectroscopy,
pubmed-meshheading:14622190-Male,
pubmed-meshheading:14622190-Muscarinic Agonists,
pubmed-meshheading:14622190-Muscarinic Antagonists,
pubmed-meshheading:14622190-Neurons,
pubmed-meshheading:14622190-Pilocarpine,
pubmed-meshheading:14622190-Rats,
pubmed-meshheading:14622190-Rats, Wistar,
pubmed-meshheading:14622190-Scopolamine Hydrobromide,
pubmed-meshheading:14622190-Time Factors
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pubmed:year |
2003
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pubmed:articleTitle |
Hippocampal N-acetyl aspartate levels do not mirror neuronal cell densities in creatine-supplemented epileptic rats.
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pubmed:affiliation |
Department of Neurology II, University of Madgeburg, Germany.
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pubmed:publicationType |
Journal Article,
Comparative Study,
In Vitro,
Research Support, Non-U.S. Gov't
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