| pubmed-article:14617361 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:14617361 | lifeskim:mentions | umls-concept:C0598953 | lld:lifeskim |
| pubmed-article:14617361 | lifeskim:mentions | umls-concept:C1533691 | lld:lifeskim |
| pubmed-article:14617361 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:14617361 | pubmed:issue | 11 | lld:pubmed |
| pubmed-article:14617361 | pubmed:dateCreated | 2003-11-17 | lld:pubmed |
| pubmed-article:14617361 | pubmed:abstractText | The mannose 6-phosphate receptor MPR46 mediates sorting of lysosomal enzymes and recycles between the trans-Golgi network and endosomes. We characterized the retrograde transport of MPR46 from endosomes to the TGN by an in vitro transport assay using mouse fibroblast cell lines. Sulfation of a modified MPR46 upon entering the TGN is measured. The in vitro retrograde transport is time-, temperature-, ATP- and cytosol-dependent. Transport requires the SNARE proteins Vti1a and Syntaxin 16 and the Rab family member Rab6. The transport is sensitive to GTP gamma S, brefeldin A and independent of TIP47. These data indicate that MPR46 follows an early endosome-to-TGN route. Transport is inhibited by MPR46 tail peptide comprising the acidic cluster-di-leucine sorting motif to which adaptor proteins AP-1 and AP-3 bind. Transport depends on cytosolic AP-3, but not on cytosolic AP-1. Residual membrane-associated AP-1 may have masked a requirement for cytosolic AP-1. The competence of membranes from AP-1-deficient cells for endosome-to-TGN transport in vitro was severely compromised. | lld:pubmed |
| pubmed-article:14617361 | pubmed:language | eng | lld:pubmed |
| pubmed-article:14617361 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14617361 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:14617361 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:14617361 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:14617361 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:14617361 | pubmed:issn | 1398-9219 | lld:pubmed |
| pubmed-article:14617361 | pubmed:author | pubmed-author:RaderJ IJI | lld:pubmed |
| pubmed-article:14617361 | pubmed:author | pubmed-author:MedigeshiGuru... | lld:pubmed |
| pubmed-article:14617361 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:14617361 | pubmed:volume | 4 | lld:pubmed |
| pubmed-article:14617361 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:14617361 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:14617361 | pubmed:pagination | 802-11 | lld:pubmed |
| pubmed-article:14617361 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
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| pubmed-article:14617361 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:14617361 | pubmed:articleTitle | Characterization of the in vitro retrograde transport of MPR46. | lld:pubmed |
| pubmed-article:14617361 | pubmed:affiliation | Georg-August Universität Göttingen, Zentrum für Biochemie und Molekulare Zellbiologie, Biochemie II, Heinrich-Düker-Weg-12, D-37073 Goettingen, Germany. | lld:pubmed |
| pubmed-article:14617361 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:14617361 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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