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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
2003-11-17
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pubmed:abstractText |
The mannose 6-phosphate receptor MPR46 mediates sorting of lysosomal enzymes and recycles between the trans-Golgi network and endosomes. We characterized the retrograde transport of MPR46 from endosomes to the TGN by an in vitro transport assay using mouse fibroblast cell lines. Sulfation of a modified MPR46 upon entering the TGN is measured. The in vitro retrograde transport is time-, temperature-, ATP- and cytosol-dependent. Transport requires the SNARE proteins Vti1a and Syntaxin 16 and the Rab family member Rab6. The transport is sensitive to GTP gamma S, brefeldin A and independent of TIP47. These data indicate that MPR46 follows an early endosome-to-TGN route. Transport is inhibited by MPR46 tail peptide comprising the acidic cluster-di-leucine sorting motif to which adaptor proteins AP-1 and AP-3 bind. Transport depends on cytosolic AP-3, but not on cytosolic AP-1. Residual membrane-associated AP-1 may have masked a requirement for cytosolic AP-1. The competence of membranes from AP-1-deficient cells for endosome-to-TGN transport in vitro was severely compromised.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adaptor Protein Complex 1,
http://linkedlifedata.com/resource/pubmed/chemical/Adaptor Protein Complex 3,
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Biological Markers,
http://linkedlifedata.com/resource/pubmed/chemical/Brefeldin A,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Intracellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/PLIN3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Pregnancy Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Synthesis Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, IGF Type 2,
http://linkedlifedata.com/resource/pubmed/chemical/Vesicular Transport Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
1398-9219
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
4
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
802-11
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:14617361-Adaptor Protein Complex 1,
pubmed-meshheading:14617361-Adaptor Protein Complex 3,
pubmed-meshheading:14617361-Adenosine Triphosphate,
pubmed-meshheading:14617361-Animals,
pubmed-meshheading:14617361-Biological Markers,
pubmed-meshheading:14617361-Biological Transport,
pubmed-meshheading:14617361-Brefeldin A,
pubmed-meshheading:14617361-Cell Membrane,
pubmed-meshheading:14617361-Cells, Cultured,
pubmed-meshheading:14617361-Cytoplasm,
pubmed-meshheading:14617361-DNA-Binding Proteins,
pubmed-meshheading:14617361-Endosomes,
pubmed-meshheading:14617361-Fibroblasts,
pubmed-meshheading:14617361-Intracellular Signaling Peptides and Proteins,
pubmed-meshheading:14617361-Mice,
pubmed-meshheading:14617361-Peptides,
pubmed-meshheading:14617361-Pregnancy Proteins,
pubmed-meshheading:14617361-Protein Synthesis Inhibitors,
pubmed-meshheading:14617361-Protein Transport,
pubmed-meshheading:14617361-Receptor, IGF Type 2,
pubmed-meshheading:14617361-Temperature,
pubmed-meshheading:14617361-Vesicular Transport Proteins,
pubmed-meshheading:14617361-trans-Golgi Network
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pubmed:year |
2003
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pubmed:articleTitle |
Characterization of the in vitro retrograde transport of MPR46.
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pubmed:affiliation |
Georg-August Universität Göttingen, Zentrum für Biochemie und Molekulare Zellbiologie, Biochemie II, Heinrich-Düker-Weg-12, D-37073 Goettingen, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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