Linked Life Data

14613721

Source:http://linkedlifedata.com/resource/pubmed/id/14613721

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2003-11-17
pubmed:abstractText
The mechanism by which asbestos fibers are fibrogenic and tumorigenic is still matter of debate. The higher pathogenicity of longer fibers has been traditionally associated with their slower clearance in respiratory airways. However, short amosite fibers, obtained by grinding longer ones, exhibited a lower potential to damage nude DNA and a lower in vitro cytotoxicity. We have thus revisited the two sets of long and short fibers in order to compare their surface properties to their activity in cell systems. In this study we report that, in human lung epithelial cells A549, long amosite fibers, more effectively than the short ones, initiate free radical reactions, inhibit the glucose 6-phosphate dehydrogenase activity and the pentose phosphate pathway, decrease the intracellular level of reduced glutathione, and increase the generation of thiobarbituric acid reactive substances and the leakage of lactate dehydrogenase in the extracellular medium. These results suggest that the shortening of fibers by prolonged milling affects not only their biopersistence, but also their surface properties, hence their interaction with cellular metabolism. Our data provide also a mechanism by which asbestos fibers inhibit the pentose phosphate pathway, i.e., via the oxidative inhibition of glucose 6-phosphate dehydrogenase, which is prevented by reduced glutathione.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0041-008X
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
193
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
106-15
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:14613721-Animals, pubmed-meshheading:14613721-Asbestos, Amosite, pubmed-meshheading:14613721-Blotting, Western, pubmed-meshheading:14613721-Carcinogens, pubmed-meshheading:14613721-Electron Spin Resonance Spectroscopy, pubmed-meshheading:14613721-Epithelial Cells, pubmed-meshheading:14613721-Free Radicals, pubmed-meshheading:14613721-Glucosephosphate Dehydrogenase, pubmed-meshheading:14613721-Glutathione, pubmed-meshheading:14613721-Humans, pubmed-meshheading:14613721-L-Lactate Dehydrogenase, pubmed-meshheading:14613721-Lung, pubmed-meshheading:14613721-Mice, pubmed-meshheading:14613721-Mineral Fibers, pubmed-meshheading:14613721-Oxidation-Reduction, pubmed-meshheading:14613721-Pentose Phosphate Pathway, pubmed-meshheading:14613721-RNA, pubmed-meshheading:14613721-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:14613721-Surface Properties, pubmed-meshheading:14613721-Thiobarbituric Acid Reactive Substances
pubmed:year
2003
pubmed:articleTitle
Long and short fiber amosite asbestos alters at a different extent the redox metabolism in human lung epithelial cells.
pubmed:affiliation
Interdepartmental Center G. Scansetti for Studies on Asbestos and other Toxic Particulates, University of Torino, Torino, Italy.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't