Source:http://linkedlifedata.com/resource/pubmed/id/14613720
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
2003-11-17
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pubmed:abstractText |
Cardiotoxin-III (CTX-III), a major cardiotoxin isolated from the venom of the Taiwan cobra (Naja naja atra), is a highly basic, hydrophobic, toxic protein, which can induce lysis of mononuclear cells by an unknown mechanism. This study was undertaken to investigate the effects of CTX-III on untreated and PHA-activated peripheral blood mononuclear cells (PBMCs) in vitro. The results show that treatment of PHA-activated lymphocytes with CTX-III (10 microg/ml) induced apoptosis and depletion of the CD8(+) population. In both untreated and PHA-treated lymphocytes, interferon-gamma production was dramatically reduced and interleukin-2 (IL-2) production was moderately reduced by CTX-III treatment. In PHA-activated lymphocytes, CD4 expression was increased, whereas CD8 and IL-2R beta chain (CD25) expression were decreased. In contrast, CTX-III had no effect on the viability of PHA-activated monocytes but significantly enhanced their tumor necrosis factor-alpha production. These results show that CTX-III selectively enhanced activation-induced apoptosis in CD8(+) T cells. CTX-III was found to bind to the cell membrane of PHA-stimulated PBMCs, and three CTX-III-binding proteins, with molecular weights of 92, 77, and 68 kDa, were identified. We therefore propose that CTX-III interacts with one or more cell surface proteins and initiates a signal pathway causing functional changes. These findings provide an insight into the immunomodulatory properties of CTX-III and suggest a novel method for the selective induction of apoptosis in CD8(+) T lymphocytes.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1,5-bis(2-methoxy-4-nitro-5-sulfophe...,
http://linkedlifedata.com/resource/pubmed/chemical/Cobra Cardiotoxin Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cobra Venoms,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Formazans,
http://linkedlifedata.com/resource/pubmed/chemical/Phytohemagglutinins,
http://linkedlifedata.com/resource/pubmed/chemical/cardiotoxin III, Naja naja atra
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0041-008X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
193
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
97-105
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:14613720-Adult,
pubmed-meshheading:14613720-Apoptosis,
pubmed-meshheading:14613720-CD8-Positive T-Lymphocytes,
pubmed-meshheading:14613720-Cell Cycle,
pubmed-meshheading:14613720-Cell Line, Tumor,
pubmed-meshheading:14613720-Cobra Cardiotoxin Proteins,
pubmed-meshheading:14613720-Cobra Venoms,
pubmed-meshheading:14613720-Cytokines,
pubmed-meshheading:14613720-Female,
pubmed-meshheading:14613720-Flow Cytometry,
pubmed-meshheading:14613720-Formazans,
pubmed-meshheading:14613720-Humans,
pubmed-meshheading:14613720-In Situ Nick-End Labeling,
pubmed-meshheading:14613720-Lymphocyte Activation,
pubmed-meshheading:14613720-Male,
pubmed-meshheading:14613720-Monocytes,
pubmed-meshheading:14613720-Phytohemagglutinins
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pubmed:year |
2003
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pubmed:articleTitle |
Cardiotoxin-III selectively enhances activation-induced apoptosis of human CD8+ T lymphocytes.
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pubmed:affiliation |
Department of Cosmetic Science, Chung Hwa College of Medical Technology, Tainan 717 Taiwan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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