Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
23
pubmed:dateCreated
2003-11-12
pubmed:abstractText
Splicing of the Saccharomyces cerevisiae mitochondrial DNA group II intron aI2 depends on the intron-encoded 62-kDa reverse transcriptase-maturase protein (p62). In wild-type strains, p62 remains associated with the excised intron lariat RNA in ribonucleoprotein (RNP) particles that are essential for intron homing. Studies of a bacterial group II intron showed that the DIVa substructure of intron domain IV is a high-affinity binding site for its maturase. Here we first present in vitro evidence extending that conclusion to aI2. Then, experiments with aI2 DIVa mutant strains show that the binding of p62 to DIVa is not essential for aI2 splicing in vivo but is essential for homing. Because aI2 splicing in the DIVa mutant strains remains maturase dependent, splicing must rely on other RNA-protein contacts. The p62 that accumulates in the mutant strains has reverse transcriptase activity, but fractionation experiments at high and low salt concentrations show that it associates more weakly than the wild-type protein with endogenous mitochondrial RNAs, and that phenotype probably explains the homing defect. Replacing the DIVa of aI2 with that of the closely related intron aI1 improves in vivo splicing but not homing, indicating that DIVa contributes to the specificity of the maturase-RNA interaction needed for homing.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/14612420-10356323, http://linkedlifedata.com/resource/pubmed/commentcorrection/14612420-10368957, http://linkedlifedata.com/resource/pubmed/commentcorrection/14612420-10413481, http://linkedlifedata.com/resource/pubmed/commentcorrection/14612420-10481026, http://linkedlifedata.com/resource/pubmed/commentcorrection/14612420-10488339, http://linkedlifedata.com/resource/pubmed/commentcorrection/14612420-10917534, http://linkedlifedata.com/resource/pubmed/commentcorrection/14612420-11046140, http://linkedlifedata.com/resource/pubmed/commentcorrection/14612420-11222775, http://linkedlifedata.com/resource/pubmed/commentcorrection/14612420-11743002, http://linkedlifedata.com/resource/pubmed/commentcorrection/14612420-11827468, http://linkedlifedata.com/resource/pubmed/commentcorrection/14612420-12051838, http://linkedlifedata.com/resource/pubmed/commentcorrection/14612420-12470950, http://linkedlifedata.com/resource/pubmed/commentcorrection/14612420-12495443, http://linkedlifedata.com/resource/pubmed/commentcorrection/14612420-12527773, http://linkedlifedata.com/resource/pubmed/commentcorrection/14612420-1569932, http://linkedlifedata.com/resource/pubmed/commentcorrection/14612420-1579468, http://linkedlifedata.com/resource/pubmed/commentcorrection/14612420-1972561, http://linkedlifedata.com/resource/pubmed/commentcorrection/14612420-2440339, http://linkedlifedata.com/resource/pubmed/commentcorrection/14612420-2834080, http://linkedlifedata.com/resource/pubmed/commentcorrection/14612420-3297351, http://linkedlifedata.com/resource/pubmed/commentcorrection/14612420-3405208, http://linkedlifedata.com/resource/pubmed/commentcorrection/14612420-6188839, http://linkedlifedata.com/resource/pubmed/commentcorrection/14612420-6253454, http://linkedlifedata.com/resource/pubmed/commentcorrection/14612420-6254986, http://linkedlifedata.com/resource/pubmed/commentcorrection/14612420-6986989, http://linkedlifedata.com/resource/pubmed/commentcorrection/14612420-6986990, http://linkedlifedata.com/resource/pubmed/commentcorrection/14612420-7011381, http://linkedlifedata.com/resource/pubmed/commentcorrection/14612420-7512259, http://linkedlifedata.com/resource/pubmed/commentcorrection/14612420-7537853, http://linkedlifedata.com/resource/pubmed/commentcorrection/14612420-7538669, http://linkedlifedata.com/resource/pubmed/commentcorrection/14612420-7574489, http://linkedlifedata.com/resource/pubmed/commentcorrection/14612420-7585955, http://linkedlifedata.com/resource/pubmed/commentcorrection/14612420-7681727, http://linkedlifedata.com/resource/pubmed/commentcorrection/14612420-7893710, http://linkedlifedata.com/resource/pubmed/commentcorrection/14612420-8029012, http://linkedlifedata.com/resource/pubmed/commentcorrection/14612420-8346031, http://linkedlifedata.com/resource/pubmed/commentcorrection/14612420-8655550, http://linkedlifedata.com/resource/pubmed/commentcorrection/14612420-8965700, http://linkedlifedata.com/resource/pubmed/commentcorrection/14612420-8965729, http://linkedlifedata.com/resource/pubmed/commentcorrection/14612420-9118229, http://linkedlifedata.com/resource/pubmed/commentcorrection/14612420-9362497, http://linkedlifedata.com/resource/pubmed/commentcorrection/14612420-9727488
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0270-7306
pubmed:author
pubmed:issnType
Print
pubmed:volume
23
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
8809-19
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
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