Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
22
pubmed:dateCreated
2003-11-11
pubmed:abstractText
The genome of Saccharomyces cerevisiae contains 35 members of the mitochondrial carrier protein family, most of which have not yet been functionally identified. Here the identification of the mitochondrial carrier for S-adenosylmethionine (SAM) Sam5p is described. The corresponding gene has been overexpressed in bacteria and the protein has been reconstituted into phospholipid vesicles and identified by its transport properties. In confirmation of its identity, (i) the Sam5p-GFP protein was found to be targeted to mitochondria; (ii) the cells lacking the gene for this carrier showed auxotrophy for biotin (which is synthesized in the mitochondria by the SAM-requiring Bio2p) on fermentable carbon sources and a petite phenotype on non-fermentable substrates; and (iii) both phenotypes of the knock-out mutant were overcome by expressing the cytosolic SAM synthetase (Sam1p) inside the mitochondria.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/14609944-10207067, http://linkedlifedata.com/resource/pubmed/commentcorrection/14609944-10835343, http://linkedlifedata.com/resource/pubmed/commentcorrection/14609944-11013234, http://linkedlifedata.com/resource/pubmed/commentcorrection/14609944-11054823, http://linkedlifedata.com/resource/pubmed/commentcorrection/14609944-11153271, http://linkedlifedata.com/resource/pubmed/commentcorrection/14609944-11566870, http://linkedlifedata.com/resource/pubmed/commentcorrection/14609944-11566871, http://linkedlifedata.com/resource/pubmed/commentcorrection/14609944-11752249, http://linkedlifedata.com/resource/pubmed/commentcorrection/14609944-11768764, http://linkedlifedata.com/resource/pubmed/commentcorrection/14609944-11852914, http://linkedlifedata.com/resource/pubmed/commentcorrection/14609944-11867542, http://linkedlifedata.com/resource/pubmed/commentcorrection/14609944-11897791, http://linkedlifedata.com/resource/pubmed/commentcorrection/14609944-11914276, http://linkedlifedata.com/resource/pubmed/commentcorrection/14609944-12134146, http://linkedlifedata.com/resource/pubmed/commentcorrection/14609944-12171066, http://linkedlifedata.com/resource/pubmed/commentcorrection/14609944-12237468, http://linkedlifedata.com/resource/pubmed/commentcorrection/14609944-12411483, http://linkedlifedata.com/resource/pubmed/commentcorrection/14609944-12631327, http://linkedlifedata.com/resource/pubmed/commentcorrection/14609944-12631701, http://linkedlifedata.com/resource/pubmed/commentcorrection/14609944-1413997, http://linkedlifedata.com/resource/pubmed/commentcorrection/14609944-1636720, http://linkedlifedata.com/resource/pubmed/commentcorrection/14609944-1903502, http://linkedlifedata.com/resource/pubmed/commentcorrection/14609944-2005794, http://linkedlifedata.com/resource/pubmed/commentcorrection/14609944-2659436, http://linkedlifedata.com/resource/pubmed/commentcorrection/14609944-3001645, http://linkedlifedata.com/resource/pubmed/commentcorrection/14609944-3316224, http://linkedlifedata.com/resource/pubmed/commentcorrection/14609944-4562409, http://linkedlifedata.com/resource/pubmed/commentcorrection/14609944-6363386, http://linkedlifedata.com/resource/pubmed/commentcorrection/14609944-7941739, http://linkedlifedata.com/resource/pubmed/commentcorrection/14609944-8117110, http://linkedlifedata.com/resource/pubmed/commentcorrection/14609944-8266080, http://linkedlifedata.com/resource/pubmed/commentcorrection/14609944-8349643, http://linkedlifedata.com/resource/pubmed/commentcorrection/14609944-8363582, http://linkedlifedata.com/resource/pubmed/commentcorrection/14609944-8592459, http://linkedlifedata.com/resource/pubmed/commentcorrection/14609944-9178508, http://linkedlifedata.com/resource/pubmed/commentcorrection/14609944-9224731, http://linkedlifedata.com/resource/pubmed/commentcorrection/14609944-9483801, http://linkedlifedata.com/resource/pubmed/commentcorrection/14609944-9514746, http://linkedlifedata.com/resource/pubmed/commentcorrection/14609944-9632789, http://linkedlifedata.com/resource/pubmed/commentcorrection/14609944-9712911
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0261-4189
pubmed:author
pubmed:issnType
Print
pubmed:day
17
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5975-82
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Identification and functional reconstitution of yeast mitochondrial carrier for S-adenosylmethionine.
pubmed:affiliation
Department of Pharmaco-Biology, Laboratory of Biochemistry and Molecular Biology, University of Bari, Bari, Italy.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't