Linked Life Data

14609564

Source:http://linkedlifedata.com/resource/pubmed/id/14609564

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2003-11-11
pubmed:abstractText
Our study aimed to investigate whether transforming growth factor-beta2 (TGF-beta2), increased in the aqueous humor of eyes with primary open angle glaucoma (POAG), can affect factors responsible for the activity of matrix metalloproteinases (MMPs) in human trabecular cell cultures. With this goal in mind cultures of human trabecular meshwork (hTM) cells derived from 8 donors were treated with TGF-beta2 for 24, 36 and 48 hr. Influence of TGF-beta2 on expression of MMP-2, MMP-9, membrane type 1-MMP (MT1-MMP) and plasminogen activator inhibitor-1 (PAI-1) was examined using RT-PCR, Northern Blot, Western Blot and zymography. The influence of TGF-beta2 treatment on PAI-1 expression was also investigated using immunohistochemistry. It appeared that treatment with TGF-beta2 significantly increased expression of the proform of MMP-2, whereas the active form was not detectable. MMP-9 and MT1-MMP expression were not influenced by TGF-beta2 treatment. There was, however, a significant increase in PAI-1 expression. To investigate whether transformation of the proform of MMP-2 to the active form was inhibited by PAI-1, the influence of treatment with TGF-beta2 and a PAI-1 neutralizing antibody on MMP-2 was investigated using zymogram method. With this treatment protocol the active form of MMP-2 was clearly visible, indicating that TGF-beta2 enhancement of the PAI-1-expression decreases MMP activity. Inhibition of MMP activity through elevated levels of TGF-beta2 might contribute to the increase in ECM in the trabecular meshwork of glaucomatous eyes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0014-4835
pubmed:author
pubmed:issnType
Print
pubmed:volume
77
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
757-65
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:14609564-Adult, pubmed-meshheading:14609564-Aged, pubmed-meshheading:14609564-Blotting, Northern, pubmed-meshheading:14609564-Blotting, Western, pubmed-meshheading:14609564-Cells, Cultured, pubmed-meshheading:14609564-Gelatin, pubmed-meshheading:14609564-Humans, pubmed-meshheading:14609564-Immunohistochemistry, pubmed-meshheading:14609564-Matrix Metalloproteinase 2, pubmed-meshheading:14609564-Matrix Metalloproteinase 9, pubmed-meshheading:14609564-Matrix Metalloproteinases, pubmed-meshheading:14609564-Middle Aged, pubmed-meshheading:14609564-Plasminogen Activator Inhibitor 1, pubmed-meshheading:14609564-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:14609564-Trabecular Meshwork, pubmed-meshheading:14609564-Transforming Growth Factor beta, pubmed-meshheading:14609564-Transforming Growth Factor beta2
pubmed:year
2003
pubmed:articleTitle
The effect of TGF-beta2 on human trabecular meshwork extracellular proteolytic system.
pubmed:affiliation
Department of Anatomy II, Friedrich Alexander University, Erlangen 91054, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't