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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
2003-11-7
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pubmed:abstractText |
Assessment of cure of Trypanosoma cruzi infection by antibody seroconversion usually involves several years of follow-up. Parasitological negativity is useless for cure assessment, since even untreated patients mostly show negative results; conversely, positive tests are of great value because they indicate treatment failure. Here, PCR was used to assess the rate of specific chemotherapy failure in a well-characterized Brazilian cohort of T. cruzi-seropositive children, who were enrolled in a field trial of benznidazole (Bz) efficacy. Paired blood samples from 111 children were taken at baseline and 36 months after treatment with either Bz (n = 58) or a placebo (n = 53). DNA extraction and PCR amplification were carried out as previously described, and hybridization was performed with all PCR products. At the end of follow-up, PCR was positive for 39.6% of the patients in the Bz group versus 64.2% in the placebo group (P = 0.01). Untreated patients had a 1.6-fold-higher chance of remaining positive by PCR than those in the Bz group (P < 0.05). We conclude that PCR is a useful tool for revealing therapeutic failure of T. cruzi infection on a short-term basis.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/14605140-10072137,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14605140-10232785,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14605140-10322321,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14605140-10463685,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14605140-11562709,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14605140-11716095,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14605140-12048553,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14605140-12209329,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14605140-12354873,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14605140-1662334,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14605140-1842429,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14605140-2270100,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14605140-2517040,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14605140-2517566,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14605140-2517801,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14605140-2565018,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14605140-2830509,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14605140-7063747,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14605140-7724232,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14605140-7851750,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14605140-8246754,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14605140-8408566,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14605140-8444472,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14605140-8542987,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14605140-8761570,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14605140-8789049,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14605140-8937280,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14605140-9107023,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14605140-9571142,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14605140-9790423
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0095-1137
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
41
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5066-70
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:14605140-Animals,
pubmed-meshheading:14605140-Antibodies, Protozoan,
pubmed-meshheading:14605140-Chagas Disease,
pubmed-meshheading:14605140-Child,
pubmed-meshheading:14605140-Drug Therapy,
pubmed-meshheading:14605140-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:14605140-Humans,
pubmed-meshheading:14605140-Monitoring, Physiologic,
pubmed-meshheading:14605140-Parasitemia,
pubmed-meshheading:14605140-Polymerase Chain Reaction,
pubmed-meshheading:14605140-Trypanosoma cruzi
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pubmed:year |
2003
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pubmed:articleTitle |
PCR assay for monitoring Trypanosoma cruzi parasitemia in childhood after specific chemotherapy.
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pubmed:affiliation |
Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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