Source:http://linkedlifedata.com/resource/pubmed/id/14603441
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
2003-11-6
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pubmed:abstractText |
We have cloned a cDNA from chromosome band 17p13.3, designated as HCAP1 (HCC-associated protein 1, originally named HC56). Database searches revealed that HCAP1 shares most of its open reading frame with GEMIN4. Single nucleotide polymorphism (SNP) screening revealed a high incidence of SNP in the coding region of HCAP1 (12 SNP sites). A collection of 140 controls and 22 cases from the Qidong area was genotyped at 6 SNP sites. The 22 cases exhibited higher frequencies of minor alleles than did the controls, and 2 sites revealed significant differences between the controls and the cases. We constructed 2 haplotypes, HCAP1-N (with common alleles at 5 SNP sites) and HCAP1-M (with minor alleles at 5 SNP sites), in a mammalian expression system. Both haplotypes resulted in a remarkable reduction in colony formation and suppression of cell growth after being transfected into the human hepatocellular carcinoma (HCC) cell line. The inhibitory effect of HCAP1-N was stronger than that of HCAP1-M. Different haplotypes also resulted in different gene expression profiles in the Hep3B cell line according to an examination of 588 genes on an Atlas membrane. The expression induced by HCAP1-M caused an up-regulation of genes involved in cellular proliferation and a down-regulation of genes involved in cellular apoptosis and DNA repair. These results, in addition to the statistical data, are biological evidence that the HCAP1-M variant of HCAP1 has a reduced inhibitory effect on hepatocarcinoma cell growth and an impaired DNA repair system. This suggests that HCAP1-M may be related to cancer susceptibility.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1045-2257
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pubmed:author | |
pubmed:copyrightInfo |
Copyright 2003 Wiley-Liss, Inc.
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pubmed:issnType |
Print
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pubmed:volume |
39
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
48-58
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:14603441-Alleles,
pubmed-meshheading:14603441-Carcinoma, Hepatocellular,
pubmed-meshheading:14603441-Cell Division,
pubmed-meshheading:14603441-Cell Line, Tumor,
pubmed-meshheading:14603441-China,
pubmed-meshheading:14603441-Down-Regulation,
pubmed-meshheading:14603441-Genetic Variation,
pubmed-meshheading:14603441-Genotype,
pubmed-meshheading:14603441-Haplotypes,
pubmed-meshheading:14603441-Humans,
pubmed-meshheading:14603441-Liver Neoplasms,
pubmed-meshheading:14603441-Neoplasm Proteins,
pubmed-meshheading:14603441-Polymorphism, Single Nucleotide,
pubmed-meshheading:14603441-Up-Regulation
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pubmed:year |
2004
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pubmed:articleTitle |
Two variants of the human hepatocellular carcinoma-associated HCAP1 gene and their effect on the growth of the human liver cancer cell line Hep3B.
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pubmed:affiliation |
National Laboratory for Oncogenes and Related Genes, Cancer Institute of Shanghai Jiao Tong University, People's Republic of China.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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