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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
22
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pubmed:dateCreated |
2003-11-6
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pubmed:abstractText |
The Cre/loxP recombination system is a commonly used tool to alter the mouse genome in a conditional manner by deletion or inversion of loxP-flanked DNA segments. While Cre-mediated deletion is essentially unidirectional, inversion is reversible and therefore does not allow the stable alteration of gene function in cells that constitutively express Cre. Site-directed mutagenesis yielded a pair of asymmetric loxP sites (lox66 and lox71) that display a favorable forward reaction equilibrium. Here, we demonstrate that lox66/lox71 mediates efficient and predominantly unidirectional inversion of a switch substrate targeted to the mouse genome in combination with either inducible or cell type-specific cre-transgenes in vivo.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/14602933-10477521,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14602933-10856932,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14602933-11034213,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14602933-11224523,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14602933-11904364,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14602933-11983152,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14602933-12202778,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14602933-12354622,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14602933-12665802,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14602933-14499113,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14602933-1924327,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14602933-3457367,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14602933-7660125,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14602933-7742860,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14602933-8235657,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14602933-8387893,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14602933-8402911,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14602933-8559668,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14602933-8596914,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14602933-8698848,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14602933-9016639,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14602933-9075923,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14602933-9288963,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14602933-9323135,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14602933-9326948,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14602933-9430222
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
1362-4962
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
15
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pubmed:volume |
31
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
e140
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:14602933-Animals,
pubmed-meshheading:14602933-Attachment Sites, Microbiological,
pubmed-meshheading:14602933-B-Lymphocytes,
pubmed-meshheading:14602933-Blotting, Southern,
pubmed-meshheading:14602933-Cell Line,
pubmed-meshheading:14602933-DNA,
pubmed-meshheading:14602933-Female,
pubmed-meshheading:14602933-Flow Cytometry,
pubmed-meshheading:14602933-Gene Rearrangement,
pubmed-meshheading:14602933-Immunoglobulin Heavy Chains,
pubmed-meshheading:14602933-Integrases,
pubmed-meshheading:14602933-Male,
pubmed-meshheading:14602933-Mice,
pubmed-meshheading:14602933-Mice, Transgenic,
pubmed-meshheading:14602933-Mutation,
pubmed-meshheading:14602933-Recombination, Genetic,
pubmed-meshheading:14602933-T-Lymphocytes,
pubmed-meshheading:14602933-Viral Proteins
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pubmed:year |
2003
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pubmed:articleTitle |
Unidirectional Cre-mediated genetic inversion in mice using the mutant loxP pair lox66/lox71.
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pubmed:affiliation |
The CBR Institute for Biomedical Research, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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