Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
22
pubmed:dateCreated
2003-11-6
pubmed:abstractText
The ability to mount an immune defense against infectious microorganisms and their products, and against tumors is believed to be a direct function of lymphocyte diversity. Because the diversity of lymphocyte receptor genes is >1000-fold more diverse than the entire genome and varies between genetically identical individuals, measuring lymphocyte diversity has been a daunting challenge. We developed a novel technique for measuring lymphocyte diversity directly using gene chips. We reasoned and here demonstrate that the frequency of hybridization of nucleic acids coding for lymphocyte receptors to the oligonucleotides on a gene chip varies in direct proportion to diversity. We applied the technique to detect changes in lymphocyte diversity in mice with known B cell alterations and in persons with known T cell repertoire defects. This approach is the first to provide direct analysis of lymphocyte receptor diversity and should facilitate fundamental study of the adaptive immune system and clinical efforts to assess immunological diseases. In addition, this approach could be more broadly applied, for example to measure diversity of viral quasi-species.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/14602932-10070037, http://linkedlifedata.com/resource/pubmed/commentcorrection/14602932-10359583, http://linkedlifedata.com/resource/pubmed/commentcorrection/14602932-10542151, http://linkedlifedata.com/resource/pubmed/commentcorrection/14602932-10764648, http://linkedlifedata.com/resource/pubmed/commentcorrection/14602932-11040185, http://linkedlifedata.com/resource/pubmed/commentcorrection/14602932-11163227, http://linkedlifedata.com/resource/pubmed/commentcorrection/14602932-11248154, http://linkedlifedata.com/resource/pubmed/commentcorrection/14602932-11418476, http://linkedlifedata.com/resource/pubmed/commentcorrection/14602932-12149467, http://linkedlifedata.com/resource/pubmed/commentcorrection/14602932-12209519, http://linkedlifedata.com/resource/pubmed/commentcorrection/14602932-12492592, http://linkedlifedata.com/resource/pubmed/commentcorrection/14602932-2477243, http://linkedlifedata.com/resource/pubmed/commentcorrection/14602932-7734044, http://linkedlifedata.com/resource/pubmed/commentcorrection/14602932-8347558, http://linkedlifedata.com/resource/pubmed/commentcorrection/14602932-8483950, http://linkedlifedata.com/resource/pubmed/commentcorrection/14602932-8658139, http://linkedlifedata.com/resource/pubmed/commentcorrection/14602932-8841456, http://linkedlifedata.com/resource/pubmed/commentcorrection/14602932-9550375, http://linkedlifedata.com/resource/pubmed/commentcorrection/14602932-9826720
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1362-4962
pubmed:author
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
31
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
e139
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:14602932-Adult, pubmed-meshheading:14602932-Animals, pubmed-meshheading:14602932-Antibody Diversity, pubmed-meshheading:14602932-Gene Rearrangement, pubmed-meshheading:14602932-Humans, pubmed-meshheading:14602932-Immunoglobulin Heavy Chains, pubmed-meshheading:14602932-Jurkat Cells, pubmed-meshheading:14602932-Lymphocytes, pubmed-meshheading:14602932-Mice, pubmed-meshheading:14602932-Mice, Inbred C57BL, pubmed-meshheading:14602932-Mice, Knockout, pubmed-meshheading:14602932-Middle Aged, pubmed-meshheading:14602932-Mutation, pubmed-meshheading:14602932-Oligonucleotide Array Sequence Analysis, pubmed-meshheading:14602932-RNA, pubmed-meshheading:14602932-RNA, Complementary, pubmed-meshheading:14602932-Receptors, Antigen, pubmed-meshheading:14602932-Receptors, Antigen, B-Cell, pubmed-meshheading:14602932-Receptors, Antigen, T-Cell, pubmed-meshheading:14602932-Reproducibility of Results
pubmed:year
2003
pubmed:articleTitle
Direct measurement of lymphocyte receptor diversity.
pubmed:affiliation
Transplantation Biology Program, Department of Physiology, Mayo Clinic, Rochester, Minnesota, MN 55905, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.