Linked Life Data

14602787

Source:http://linkedlifedata.com/resource/pubmed/id/14602787

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2003-11-6
pubmed:abstractText
We determined whole-body insulin sensitivity, long-chain fatty acyl coenzyme A (LCACoA) content, skeletal muscle triglyceride (TG(m)) concentration, fatty acid transporter protein content, and oxidative enzyme activity in eight patients with type 2 diabetes (TYPE 2); six healthy control subjects matched for age (OLD), body mass index, percentage of body fat, and maximum pulmonary O(2) uptake; nine well-trained athletes (TRAINED); and four age-matched controls (YOUNG). Muscle biopsies from the vastus lateralis were taken before and after a 2-h euglycemic-hyperinsulinemic clamp. Oxidative enzyme activities, fatty acid transporters (FAT/CD36 and FABPpm), and TG(m) were measured from basal muscle samples, and total LCACoA content was determined before and after insulin stimulation. Whole-body insulin-stimulated glucose uptake was lower in TYPE 2 (P < 0.05) than in OLD, YOUNG, and TRAINED. TG(m) was elevated in TYPE 2 compared with all other groups (P < 0.05). However, both basal and insulin-stimulated skeletal muscle LCACoA content were similar. Basal citrate synthase activity was higher in TRAINED (P < 0.01), whereas beta-hydroxyacyl CoA dehydrogenase activity was higher in TRAINED compared with TYPE 2 and OLD. There was a significant relationship between the oxidative capacity of skeletal muscle and insulin sensitivity (citrate synthase, r = 0.71, P < 0.001; beta-hydroxyacyl CoA dehydrogenase, r = 0.61, P = 0.001). No differences were found in FAT/CD36 protein content between groups. In contrast, FABPpm protein was lower in OLD compared with TYPE 2 and YOUNG (P < 0.05). In conclusion, despite markedly elevated skeletal muscle TG(m) in type 2 diabetic patients and strikingly different levels of whole-body glucose disposal, both basal and insulin-stimulated LCACoA content were similar across groups. Furthermore, skeletal muscle oxidative capacity was a better predictor of insulin sensitivity than either TG(m) concentration or long-chain fatty acyl CoA content.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0021-972X
pubmed:author
pubmed:issnType
Print
pubmed:volume
88
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5444-51
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:14602787-Adult, pubmed-meshheading:14602787-Antigens, CD36, pubmed-meshheading:14602787-Biological Markers, pubmed-meshheading:14602787-Body Mass Index, pubmed-meshheading:14602787-Carrier Proteins, pubmed-meshheading:14602787-Diabetes Mellitus, Type 2, pubmed-meshheading:14602787-Fatty Acid-Binding Proteins, pubmed-meshheading:14602787-Female, pubmed-meshheading:14602787-Glucose, pubmed-meshheading:14602787-Glucose Clamp Technique, pubmed-meshheading:14602787-Humans, pubmed-meshheading:14602787-Insulin Resistance, pubmed-meshheading:14602787-Lipid Metabolism, pubmed-meshheading:14602787-Male, pubmed-meshheading:14602787-Membrane Glycoproteins, pubmed-meshheading:14602787-Middle Aged, pubmed-meshheading:14602787-Muscle, Skeletal, pubmed-meshheading:14602787-Neoplasm Proteins, pubmed-meshheading:14602787-Organic Anion Transporters, pubmed-meshheading:14602787-Oxidative Stress, pubmed-meshheading:14602787-Predictive Value of Tests, pubmed-meshheading:14602787-Tumor Suppressor Proteins
pubmed:year
2003
pubmed:articleTitle
Muscle oxidative capacity is a better predictor of insulin sensitivity than lipid status.
pubmed:affiliation
Exercise Metabolism Group, School of Medical Sciences, RMIT University, Bundoora, Victoria 3083, Australia.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't