Linked Life Data

14602648

Source:http://linkedlifedata.com/resource/pubmed/id/14602648

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2003-11-6
pubmed:abstractText
As part of an effort to develop detectors for selected species of bacterial spores, we screened phage display peptide libraries for 7- and 12-mer peptides that bind tightly to spores of Bacillus subtilis. All of the peptides isolated contained the sequence Asn-His-Phe-Leu at the amino terminus and exhibited clear preferences for other amino acids, especially Pro, at positions 5 to 7. We demonstrated that the sequence Asn-His-Phe-Leu-Pro (but not Asn-His-Phe-Leu) was sufficient for tight spore binding. We observed equal 7-mer peptide binding to spores of B. subtilis and its most closely related species, Bacillus amyloliquefaciens, and slightly weaker binding to spores of the closely related species Bacillus globigii. These three species comprise one branch on the Bacillus phylogenetic tree. We did not detect peptide binding to spores of several Bacillus species located on adjacent and nearby branches of the phylogenetic tree nor to vegetative cells of B. subtilis. The sequence Asn-His-Phe-Leu-Pro was used to identify B. subtilis proteins that may employ this peptide for docking to the outer surface of the forespore during spore coat assembly and/or maturation. One such protein, SpsC, appears to be involved in the synthesis of polysaccharide on the spore coat. SpsC contains the Asn-His-Phe-Leu-Pro sequence at positions 6 to 10, and the first five residues of SpsC apparently must be removed to allow spore binding. Finally, we discuss the use of peptide ligands for bacterial detection and the use of short peptide sequences for targeting proteins during spore formation.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/14602648-10350455, http://linkedlifedata.com/resource/pubmed/commentcorrection/14602648-10610808, http://linkedlifedata.com/resource/pubmed/commentcorrection/14602648-10974126, http://linkedlifedata.com/resource/pubmed/commentcorrection/14602648-11040425, http://linkedlifedata.com/resource/pubmed/commentcorrection/14602648-11514528, http://linkedlifedata.com/resource/pubmed/commentcorrection/14602648-11884407, http://linkedlifedata.com/resource/pubmed/commentcorrection/14602648-12088650, http://linkedlifedata.com/resource/pubmed/commentcorrection/14602648-12618454, http://linkedlifedata.com/resource/pubmed/commentcorrection/14602648-14240968, http://linkedlifedata.com/resource/pubmed/commentcorrection/14602648-1708381, http://linkedlifedata.com/resource/pubmed/commentcorrection/14602648-185959, http://linkedlifedata.com/resource/pubmed/commentcorrection/14602648-1917883, http://linkedlifedata.com/resource/pubmed/commentcorrection/14602648-2517297, http://linkedlifedata.com/resource/pubmed/commentcorrection/14602648-4963765, http://linkedlifedata.com/resource/pubmed/commentcorrection/14602648-7592393, http://linkedlifedata.com/resource/pubmed/commentcorrection/14602648-822351, http://linkedlifedata.com/resource/pubmed/commentcorrection/14602648-8464402
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0099-2240
pubmed:author
pubmed:issnType
Print
pubmed:volume
69
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6841-7
pubmed:dateRevised
2010-9-20
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Peptide ligands that bind selectively to spores of Bacillus subtilis and closely related species.
pubmed:affiliation
Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't