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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
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pubmed:dateCreated |
1993-1-12
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pubmed:abstractText |
The active form of purified mitochondrial nicotinamide nucleotide transhydrogenase from beef heart was investigated by crosslinking with dimethylsuberimidate and SDS-PAGE, with or without pretreatment with the inactivating detergent Triton X-100. In the absence of detergent, crosslinked isomers of the dimeric form of 208-235 kDa were obtained. Addition of detergent led to the simultaneous loss of the dimers and the bulk of the activity. Removal of the detergent led to a partial restoration of both activity and the dimeric forms. The results suggest that the active form is a dimer, and that the detergent-dependent conversion to the largely inactive monomer is reversible. It is proposed that the mechanism of inactivation of transhydrogenase by Triton X-100 involves a disruption of essential hydrophobic interactions between the membrane-spanning regions of the monomers.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Dec
|
pubmed:issn |
0145-479X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
24
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
611-5
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:1459991-Animals,
pubmed-meshheading:1459991-Cattle,
pubmed-meshheading:1459991-Dimethyl Suberimidate,
pubmed-meshheading:1459991-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:1459991-Enzyme Activation,
pubmed-meshheading:1459991-Kinetics,
pubmed-meshheading:1459991-Mitochondria, Heart,
pubmed-meshheading:1459991-NADP Transhydrogenases,
pubmed-meshheading:1459991-Structure-Activity Relationship
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pubmed:year |
1992
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pubmed:articleTitle |
Correlation between active form and dimeric structure of mitochondrial nicotinamide nucleotide transhydrogenase from beef heart.
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pubmed:affiliation |
Department of Molecular Biology, University of Stockholm, Sweden.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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