Linked Life Data

14599476

Source:http://linkedlifedata.com/resource/pubmed/id/14599476

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5-6
pubmed:dateCreated
2003-11-5
pubmed:abstractText
We report the phototoxicity of meso-tetrakis(4-sulphonatophenyl)porphine (TPPS4) and zinc metallocomplex (ZnTPPS4) sensitizers in the presence or absence of 2-hydroxypropyl-beta-cyclodextrin (HP-beta-CD) on G361human melanoma cells. Morphological changes in cell cultures have been evaluated using inversion fluorescent microscope and image analysis. Viability of cells was determined by means of molecular probes for fluorescence microscopy (LIVE/DEAD kit- double staining with Calcein AM and Ethidium Homodimer). The quantitative changes of cell viability in relation to sensitizers concentrations and irradiation doses were proved by fluorometric measurement with fluoroscan Ascent. We found that the most effective sensitizer is ZnTPPS4 bound to HP-beta-CD, since the IC50 value was 12.5 g/ml at the dose of light radiation of 10 J/cm2.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0887-2333
pubmed:author
pubmed:issnType
Print
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
775-8
pubmed:dateRevised
2009-4-10
pubmed:meshHeading
pubmed:articleTitle
In vitro toxicity testing of supramolecular sensitizers for photodynamic therapy.
pubmed:affiliation
Department of Biophysics, Faculty of Medicine, Laboratory of Growth Regulators, Palacky University, Hnívotínská 3, 775 15 Olomouc, Czech Republic. kol@tunw.upol.cz
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't