Linked Life Data

14597729

Source:http://linkedlifedata.com/resource/pubmed/id/14597729

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2004-2-5
pubmed:abstractText
There is evidence for a beneficial effect of trefoil peptides in animal models of gastric damage and intestinal inflammation, but the optimal treatment strategy and the mechanistic basis have not been explored thoroughly. It has been suggested that these proteins may modulate the inflammatory response. The aims of this study were to compare the protective and curative value of systemic and topical trefoil factor family (TFF)2 administration in dextran sulfate sodium-induced experimental colitis and to investigate the relationship between the therapeutic effects of TFF2 and modulation of leukocyte recruitment and expression of cell adhesion molecules. Clinical and morphologic severity of colitis was evaluated at the end of the study (Day 10). Leukocyte-endothelial cell interactions were determined in colonic venules by fluorescence intravital microscopy. The expression of cell adhesion molecules vascular cell adhesion molecule 1 (VCAM-1) and mucosal addressin cell adhesion molecule 1 (MAdCAM-1) was measured by the dual radiolabeled monoclonal antibody technique. Pretreatment with TFF2 by subcutaneous or intracolonic (ic) route ameliorated the clinical course of colitis, and the luminal route had a significantly superior effect. This beneficial effect was correlated with significant reductions in endothelial VCAM-1 but not MAdCAM-1 expression and leukocyte adhesion to intestinal venules, which returned to levels similar to those of controls. In established colitis, ic TFF2 treatment did not modify the severity of colonic lesions. In conclusion, TFF2 is useful in the treatment of colitis, and topical administration is superior to the systemic route. Reduction in adhesion molecule expression and leukocyte recruitment into the inflamed intestine contributes to the beneficial effect of this treatment.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0741-5400
pubmed:author
pubmed:issnType
Print
pubmed:volume
75
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
214-23
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:14597729-Animals, pubmed-meshheading:14597729-Cell Adhesion Molecules, pubmed-meshheading:14597729-Cell Communication, pubmed-meshheading:14597729-Chemotaxis, Leukocyte, pubmed-meshheading:14597729-Colitis, pubmed-meshheading:14597729-Colon, pubmed-meshheading:14597729-Drug Administration Routes, pubmed-meshheading:14597729-Drug Evaluation, Preclinical, pubmed-meshheading:14597729-Endothelium, Vascular, pubmed-meshheading:14597729-Immunoglobulins, pubmed-meshheading:14597729-Leukocytes, pubmed-meshheading:14597729-Mice, pubmed-meshheading:14597729-Mice, Inbred Strains, pubmed-meshheading:14597729-Mucins, pubmed-meshheading:14597729-Mucoproteins, pubmed-meshheading:14597729-Muscle Proteins, pubmed-meshheading:14597729-Peptides, pubmed-meshheading:14597729-Vascular Cell Adhesion Molecule-1, pubmed-meshheading:14597729-Venules
pubmed:year
2004
pubmed:articleTitle
Trefoil peptide TFF2 treatment reduces VCAM-1 expression and leukocyte recruitment in experimental intestinal inflammation.
pubmed:affiliation
Hospital Clínic, IDIBAPS, University of Barcelona, Villarroel 170, 08036 Barcelona, Spain. panes@medicina.ub.es
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't