Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
23
pubmed:dateCreated
2003-11-12
pubmed:abstractText
The increase in life expectancy is accompanied by the growing burden of chronic diseases. Hearing loss is perhaps the most prevalent of all chronic diseases. In addition to age-related hearing loss, a substantial number of cases of audiological impairment are either congenital in nature or acquired during childhood. The permanence of hearing loss is mainly due to the inability of the cochlear sensory epithelium to replace lost mechanoreceptor cells, or hair cells. Generation of hair cells from a renewable source of progenitors that can be transplanted into damaged inner ears is a principal requirement for potential cell replacement therapy in this organ. Here, we present an experimental protocol that enables us to routinely create inner ear progenitors from murine embryonic stem cells in vitro. These progenitors express a comprehensive set of marker genes that define the developing inner ear, in particular the organ's developing sensory patches. We further demonstrate that cells that express markers characteristic of hair cells differentiate from embryonic stem cell-derived progenitors. Finally, we show that these progenitors integrate into the developing inner ear at sites of epithelial injury and that integrated cells start expressing hair cell markers and display hair bundles when situated in cochlear or vestibular sensory epithelia in vivo.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/14593207-10080181, http://linkedlifedata.com/resource/pubmed/commentcorrection/14593207-10226792, http://linkedlifedata.com/resource/pubmed/commentcorrection/14593207-10364557, http://linkedlifedata.com/resource/pubmed/commentcorrection/14593207-10473135, http://linkedlifedata.com/resource/pubmed/commentcorrection/14593207-10570205, http://linkedlifedata.com/resource/pubmed/commentcorrection/14593207-10660898, http://linkedlifedata.com/resource/pubmed/commentcorrection/14593207-10720333, http://linkedlifedata.com/resource/pubmed/commentcorrection/14593207-10835609, http://linkedlifedata.com/resource/pubmed/commentcorrection/14593207-10975527, http://linkedlifedata.com/resource/pubmed/commentcorrection/14593207-11110663, http://linkedlifedata.com/resource/pubmed/commentcorrection/14593207-11326082, http://linkedlifedata.com/resource/pubmed/commentcorrection/14593207-11507641, http://linkedlifedata.com/resource/pubmed/commentcorrection/14593207-11739954, http://linkedlifedata.com/resource/pubmed/commentcorrection/14593207-12072915, http://linkedlifedata.com/resource/pubmed/commentcorrection/14593207-12176325, http://linkedlifedata.com/resource/pubmed/commentcorrection/14593207-12949502, http://linkedlifedata.com/resource/pubmed/commentcorrection/14593207-12972002, http://linkedlifedata.com/resource/pubmed/commentcorrection/14593207-1689217, http://linkedlifedata.com/resource/pubmed/commentcorrection/14593207-1922362, http://linkedlifedata.com/resource/pubmed/commentcorrection/14593207-2303163, http://linkedlifedata.com/resource/pubmed/commentcorrection/14593207-2808748, http://linkedlifedata.com/resource/pubmed/commentcorrection/14593207-3897439, http://linkedlifedata.com/resource/pubmed/commentcorrection/14593207-7623109, http://linkedlifedata.com/resource/pubmed/commentcorrection/14593207-7628386, http://linkedlifedata.com/resource/pubmed/commentcorrection/14593207-8558238, http://linkedlifedata.com/resource/pubmed/commentcorrection/14593207-8815925, http://linkedlifedata.com/resource/pubmed/commentcorrection/14593207-8872459, http://linkedlifedata.com/resource/pubmed/commentcorrection/14593207-8987750, http://linkedlifedata.com/resource/pubmed/commentcorrection/14593207-9108056, http://linkedlifedata.com/resource/pubmed/commentcorrection/14593207-9256502, http://linkedlifedata.com/resource/pubmed/commentcorrection/14593207-9278160, http://linkedlifedata.com/resource/pubmed/commentcorrection/14593207-9445085
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
11
pubmed:volume
100
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
13495-500
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Generation of hair cells by stepwise differentiation of embryonic stem cells.
pubmed:affiliation
Department of Otolaryngology and Program in Neuroscience, Harvard Medical School and Eaton-Peabody Laboratory, Massachusetts Eye and Ear Infirmary, Boston, MA 02114, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't