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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1993-1-14
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pubmed:abstractText |
We describe a liquid chromatographic technique to determine L-glycerate in body fluids. The method is based on the derivatisation of the L-glycerate by incubation with lactate dehydrogenase and nicotinamide-adenine dinucleotide in the presence of phenylhydrazine. Oxidation of L-glycerate forms beta-hydroxypyruvate which is converted in turn into the related phenylhydrazone. The UV-absorbing derivative is determined using reversed-phase high performance liquid chromatography. The sensitivity was 5 mumol/l and 50 microliters of sample were required. The imprecision relative standard deviation was 4.5% and the recovery was 96.5 +/- 6.8% for L-glycerate in plasma. L-Glycerate concentrations in urine and plasma were less than 5 mumol/l in both normal individuals and patients with glycolic aciduria. In a patient with systemic oxalosis and normal plasma glycolate, plasma L-glyceric acid was 887 mumol/l.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0009-8981
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
30
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pubmed:volume |
211
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
143-53
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:1458609-Body Fluids,
pubmed-meshheading:1458609-Chromatography, High Pressure Liquid,
pubmed-meshheading:1458609-Glyceric Acids,
pubmed-meshheading:1458609-Humans,
pubmed-meshheading:1458609-Hydrogen-Ion Concentration,
pubmed-meshheading:1458609-Hyperoxaluria, Primary,
pubmed-meshheading:1458609-L-Lactate Dehydrogenase,
pubmed-meshheading:1458609-NAD,
pubmed-meshheading:1458609-Oxalates,
pubmed-meshheading:1458609-Oxalic Acid,
pubmed-meshheading:1458609-Phenylhydrazines,
pubmed-meshheading:1458609-Pyruvates,
pubmed-meshheading:1458609-Reference Values
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pubmed:year |
1992
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pubmed:articleTitle |
High-performance liquid chromatographic assay for L-glyceric acid in body fluids. Application in primary hyperoxaluria type 2.
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pubmed:affiliation |
Renal Stone Laboratory, Ospedale Mauriziano Umberto I, Turin, Italy.
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pubmed:publicationType |
Journal Article
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