| pubmed-article:14584917 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:14584917 | lifeskim:mentions | umls-concept:C0059100 | lld:lifeskim |
| pubmed-article:14584917 | lifeskim:mentions | umls-concept:C1510438 | lld:lifeskim |
| pubmed-article:14584917 | lifeskim:mentions | umls-concept:C0872187 | lld:lifeskim |
| pubmed-article:14584917 | lifeskim:mentions | umls-concept:C0439831 | lld:lifeskim |
| pubmed-article:14584917 | lifeskim:mentions | umls-concept:C1524057 | lld:lifeskim |
| pubmed-article:14584917 | pubmed:issue | 19 | lld:pubmed |
| pubmed-article:14584917 | pubmed:dateCreated | 2003-10-30 | lld:pubmed |
| pubmed-article:14584917 | pubmed:abstractText | High-throughput screening requires simple assays that give reliable quantitative results. A microplate assay was developed for reducing sugar analysis that uses a 2,2'-bicinchoninic-based protein reagent. Endo-1,4-beta-D-xylanase activity against oat spelt xylan was detected at activities of 0.002 to 0.011 IU ml(-1). The assay is linear for sugar concentrations from 0 to 86 microg ml(-1) and can also be used to assay protein concentrations (0 to 143 microg ml(-1)) on the same plate. A variety of temperatures and pH conditions can be used and, after incubation, the assay requires only one detection reagent and one heating step. | lld:pubmed |
| pubmed-article:14584917 | pubmed:language | eng | lld:pubmed |
| pubmed-article:14584917 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14584917 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:14584917 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14584917 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14584917 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14584917 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14584917 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14584917 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14584917 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14584917 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14584917 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:14584917 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:14584917 | pubmed:issn | 0141-5492 | lld:pubmed |
| pubmed-article:14584917 | pubmed:author | pubmed-author:JeffriesThoma... | lld:pubmed |
| pubmed-article:14584917 | pubmed:author | pubmed-author:KenealyWillia... | lld:pubmed |
| pubmed-article:14584917 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:14584917 | pubmed:volume | 25 | lld:pubmed |
| pubmed-article:14584917 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:14584917 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:14584917 | pubmed:pagination | 1619-23 | lld:pubmed |
| pubmed-article:14584917 | pubmed:meshHeading | pubmed-meshheading:14584917... | lld:pubmed |
| pubmed-article:14584917 | pubmed:meshHeading | pubmed-meshheading:14584917... | lld:pubmed |
| pubmed-article:14584917 | pubmed:meshHeading | pubmed-meshheading:14584917... | lld:pubmed |
| pubmed-article:14584917 | pubmed:meshHeading | pubmed-meshheading:14584917... | lld:pubmed |
| pubmed-article:14584917 | pubmed:meshHeading | pubmed-meshheading:14584917... | lld:pubmed |
| pubmed-article:14584917 | pubmed:meshHeading | pubmed-meshheading:14584917... | lld:pubmed |
| pubmed-article:14584917 | pubmed:meshHeading | pubmed-meshheading:14584917... | lld:pubmed |
| pubmed-article:14584917 | pubmed:meshHeading | pubmed-meshheading:14584917... | lld:pubmed |
| pubmed-article:14584917 | pubmed:meshHeading | pubmed-meshheading:14584917... | lld:pubmed |
| pubmed-article:14584917 | pubmed:meshHeading | pubmed-meshheading:14584917... | lld:pubmed |
| pubmed-article:14584917 | pubmed:meshHeading | pubmed-meshheading:14584917... | lld:pubmed |
| pubmed-article:14584917 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:14584917 | pubmed:articleTitle | Rapid 2,2'-bicinchoninic-based xylanase assay compatible with high throughput screening. | lld:pubmed |
| pubmed-article:14584917 | pubmed:affiliation | Institute for Microbial and Biochemical Technology, USDA Forest Service, Forest Products Laboratory, One Gifford Pinchot Drive, Madison, WI 53726-2398, USA. bkenealy@fs.fed.us | lld:pubmed |
| pubmed-article:14584917 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:14584917 | pubmed:publicationType | Evaluation Studies | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:14584917 | lld:pubmed |
