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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
19
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pubmed:dateCreated |
2003-10-30
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pubmed:abstractText |
High-throughput screening requires simple assays that give reliable quantitative results. A microplate assay was developed for reducing sugar analysis that uses a 2,2'-bicinchoninic-based protein reagent. Endo-1,4-beta-D-xylanase activity against oat spelt xylan was detected at activities of 0.002 to 0.011 IU ml(-1). The assay is linear for sugar concentrations from 0 to 86 microg ml(-1) and can also be used to assay protein concentrations (0 to 143 microg ml(-1)) on the same plate. A variety of temperatures and pH conditions can be used and, after incubation, the assay requires only one detection reagent and one heating step.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0141-5492
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
25
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1619-23
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pubmed:meshHeading |
pubmed-meshheading:14584917-Carbohydrates,
pubmed-meshheading:14584917-Endo-1,4-beta Xylanases,
pubmed-meshheading:14584917-Enzyme Activation,
pubmed-meshheading:14584917-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:14584917-Hydrogen-Ion Concentration,
pubmed-meshheading:14584917-Protein Interaction Mapping,
pubmed-meshheading:14584917-Proteins,
pubmed-meshheading:14584917-Quinolines,
pubmed-meshheading:14584917-Serum Albumin, Bovine,
pubmed-meshheading:14584917-Xylans,
pubmed-meshheading:14584917-Xylose
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pubmed:year |
2003
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pubmed:articleTitle |
Rapid 2,2'-bicinchoninic-based xylanase assay compatible with high throughput screening.
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pubmed:affiliation |
Institute for Microbial and Biochemical Technology, USDA Forest Service, Forest Products Laboratory, One Gifford Pinchot Drive, Madison, WI 53726-2398, USA. bkenealy@fs.fed.us
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pubmed:publicationType |
Journal Article,
Evaluation Studies
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