Source:http://linkedlifedata.com/resource/pubmed/id/14584050
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
2003-10-29
|
| pubmed:abstractText |
Mesenchymal stem cells (MSC) are considered as potential agents for reconstructive and gene-targeting therapies since they differentiate into various cell-lineages, exhibit an extended survival once injected into a host, and can easily be transfected with engineered DNA. MSC are essentially isolated from hematopoietic bone marrow (BM), a process that is rather invasive and may raise ethical concerns. In an attempt to find an alternative source, we evaluated whether non-hematopoietic (nh)BM recovered from femoral heads of patients undergoing hip arthroplasty contained MSC. Ex vivo, 99% of nhBM cells were CD45(+) leukocytes. After culture, leukocytes were replaced by a homogeneous layer of adherent CD45(-) CD14(-) CD34(-) CD11b(-) CD90(+) HLA-ABC(+) cells. Culture doubling time (mean = 4 days, range 1.6-6.7 days) was not correlated with patient age (27-81 years, n = 16). Amplified cultures supported long-term hematopoiesis, and could be differentiated in vitro into adipocytes and chondrocytes. Moreover, a small fraction of nhBM cells spontaneously expressed MyoD1 and formed myotubes, suggesting that myogenic differentiation also occurred. nhBM contained clonogenic cells whose frequency (1/13,000), doubling time (2.1 days), and maximal amplification (up to 10(6)-fold) were not age-related. All 14 clones analyzed (from five patients, ages 27-78 years) differentiated into at least one mesenchymal lineage, and 66% were bipotential (n = 8/12), or tripotential (n = 2/3). In conclusion, nhBM contains pluripotential mesenchymal progenitors which are similar to hematopoietic BM-derived MSC, and whose biological functions are not altered by aging. Furthermore, if MSC-based therapies hold their promises, nhBM may become the source of choice for responding to the increasing demand for MSC.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Jan
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| pubmed:issn |
0021-9541
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 2003 Wiley-Liss, Inc.
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| pubmed:issnType |
Print
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| pubmed:volume |
198
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
110-8
|
| pubmed:dateRevised |
2007-11-15
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| pubmed:meshHeading |
pubmed-meshheading:14584050-Adipocytes,
pubmed-meshheading:14584050-Adult,
pubmed-meshheading:14584050-Aged,
pubmed-meshheading:14584050-Aged, 80 and over,
pubmed-meshheading:14584050-Antigens, CD45,
pubmed-meshheading:14584050-Bone Marrow Cells,
pubmed-meshheading:14584050-Cell Differentiation,
pubmed-meshheading:14584050-Cell Division,
pubmed-meshheading:14584050-Cells, Cultured,
pubmed-meshheading:14584050-Chondrocytes,
pubmed-meshheading:14584050-Femur,
pubmed-meshheading:14584050-Hematopoiesis,
pubmed-meshheading:14584050-Humans,
pubmed-meshheading:14584050-Mesoderm,
pubmed-meshheading:14584050-Middle Aged,
pubmed-meshheading:14584050-Myoblasts,
pubmed-meshheading:14584050-Phenotype,
pubmed-meshheading:14584050-Pluripotent Stem Cells
|
| pubmed:year |
2004
|
| pubmed:articleTitle |
Non-hematopoietic human bone marrow contains long-lasting, pluripotential mesenchymal stem cells.
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| pubmed:affiliation |
Orthopedic Surgery Service, Geneva University Hospital, Geneva, Switzerland.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|