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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2003-10-27
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pubmed:abstractText |
Telomere length analysis has aroused considerable interest in biology and oncology. However, most published data are pan-genomic Southern-blot-based estimates. We developed T/C-FISH (telomere/centromere-FISH), allowing precise measurement of individual telomeres at every single chromosome arm. Metaphase preparations are co-hybridized with peptide nucleic acid probes for telomeric sequences and the chromosome 2 centromere serving as internal reference. Metaphase images are captured and karyotyped using dedicated software. A software module determines the absolute integrated fluorescence intensities of the p- and q-telomeres of each chromosome and the reference signal. Normalized data are derived by calculating the ratio of absolute telomere and reference signal intensities, and descriptive statistics are calculated. T/C-FISH detects even small differences in telomere length. Using T/C-FISH we have discovered an epigenetic process occurring in the human male postzygote or early embryo: in umbilical cord blood lymphocytes, telomeres on male Xqs are around 1100 bp shorter than female Xqs.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/14578175-10577915,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14578175-10754555,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14578175-10799705,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14578175-10906069,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14578175-11169580,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14578175-11470873,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14578175-11498580,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14578175-11595186,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14578175-11598950,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14578175-11672985,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14578175-11850022,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14578175-11850778,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14578175-12116379,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14578175-12200870,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14578175-12542689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14578175-12592375,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14578175-12661010,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14578175-7957062,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14578175-8647430,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14578175-8733138,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14578175-9207107,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14578175-9425906,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14578175-9770369
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0002-9440
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pubmed:author |
pubmed-author:BrüderleinSilkeS,
pubmed-author:ChopurianHeikoH,
pubmed-author:CilogluNeslisahN,
pubmed-author:HögelJosefJ,
pubmed-author:HaselCorneliaC,
pubmed-author:HoldenriedAlexandraA,
pubmed-author:MöllerPeterP,
pubmed-author:NielsenKirsten VangKV,
pubmed-author:PernerSvenS,
pubmed-author:PleschAndreasA,
pubmed-author:WaibelIrenaI
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pubmed:issnType |
Print
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pubmed:volume |
163
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1751-6
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:14578175-Adolescent,
pubmed-meshheading:14578175-Adult,
pubmed-meshheading:14578175-Age Factors,
pubmed-meshheading:14578175-Aged,
pubmed-meshheading:14578175-Blotting, Southern,
pubmed-meshheading:14578175-Calibration,
pubmed-meshheading:14578175-Centromere,
pubmed-meshheading:14578175-Child,
pubmed-meshheading:14578175-Child, Preschool,
pubmed-meshheading:14578175-Female,
pubmed-meshheading:14578175-Humans,
pubmed-meshheading:14578175-Image Processing, Computer-Assisted,
pubmed-meshheading:14578175-In Situ Hybridization, Fluorescence,
pubmed-meshheading:14578175-Infant,
pubmed-meshheading:14578175-Infant, Newborn,
pubmed-meshheading:14578175-Male,
pubmed-meshheading:14578175-Middle Aged,
pubmed-meshheading:14578175-Sex Factors,
pubmed-meshheading:14578175-Telomere
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pubmed:year |
2003
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pubmed:articleTitle |
Quantifying telomere lengths of human individual chromosome arms by centromere-calibrated fluorescence in situ hybridization and digital imaging.
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pubmed:affiliation |
Institute of Pathology, University Hospitals of Ulm, Albert-Einstein-Allee 11, D-89081 Ulm, Germany.
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pubmed:publicationType |
Journal Article,
Comparative Study
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