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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1993-1-11
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pubmed:databankReference |
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pubmed:abstractText |
We report the cloning and sequencing of a 1252 base pairs (bp) DNA fragment containing the bovine interleukin-6 (IL-6) gene promoter. This fragment was isolated from a bovine genomic library constructed in the lambda GEM11 vector. Comparison with human, murine and rat IL-6 gene promoters reveals a high degree of conservation of the 200 bp immediately upstream of the RNA CAP site. This region contains nucleotide stretches matching with consensus sequences recognized by transcription factors, including NF-KB, CREB and NF-IL6. A potential AP-1 binding site is found 284 nucleotides upstream of the RNA CAP site. The bovine IL-6 gene promoter cloned upstream of the bacterial chloramphenicol acetyl transferase (CAT) gene was shown to be active in bovine and ovine cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1042-5179
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
3
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
115-7
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:1457813-Animals,
pubmed-meshheading:1457813-Base Sequence,
pubmed-meshheading:1457813-Cattle,
pubmed-meshheading:1457813-Cloning, Molecular,
pubmed-meshheading:1457813-DNA,
pubmed-meshheading:1457813-Humans,
pubmed-meshheading:1457813-Interleukin-6,
pubmed-meshheading:1457813-Molecular Sequence Data,
pubmed-meshheading:1457813-Promoter Regions, Genetic,
pubmed-meshheading:1457813-Sequence Homology, Nucleic Acid
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pubmed:year |
1992
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pubmed:articleTitle |
Nucleotide sequence of the bovine interleukin-6 gene promoter.
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pubmed:affiliation |
Laboratoire de Chimie Biologique, Université Libre de Bruxelles, Rhode-St-Genèse, Belgium.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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