Linked Life Data

14577578

Source:http://linkedlifedata.com/resource/pubmed/id/14577578

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2003-10-27
pubmed:abstractText
The cardiomyopathic hamster is characterized by a naturally occurring deletion in the delta-sarcoglycan gene generating either the hypertrophic or the dilatative phenotype of cardiomyopathy. This evidence suggests that other genetic or environmental factors might concur to the pathogenesis of cardiomyopathy. The aim of the present study was to investigate on the possibility that other genes are involved in the pathogenesis of hamster cardiomyopathy. For this purpose, a series of genes of cardiomyopathic and healthy hamsters were compared by the differential display technique. The hamster cytochrome c oxidase mitochondrial subunit III (COIII) gene has been sequenced and identified as the gene upregulated in brain and skeletal muscle. The gene sequencing and restriction analysis demonstrated that a missense mutation is present in the COIII gene of hamsters exhibiting hypertrophic cardiomyopathy while no mutations were present in dilatative cardiomyopathic hamsters. The mutation was heteroplasmic and the heteroplasmy level was increased with age in skeletal muscle and heart. The ultrastructural analysis of cardiac tissue showed severe damage in the mitochondrial structure of hypertrophic but not dilatative hamster hearts. These results suggest that the pathogenesis of the cardiac damage in hypertrophic cardiomyopathic hamster may be sustained by multiple mutations exerting a cumulative effect on both structure and function of cardiac muscle.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0300-8177
pubmed:author
pubmed:issnType
Print
pubmed:volume
252
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
73-81
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:14577578-Amino Acid Sequence, pubmed-meshheading:14577578-Animals, pubmed-meshheading:14577578-Base Sequence, pubmed-meshheading:14577578-Blotting, Northern, pubmed-meshheading:14577578-Cardiomegaly, pubmed-meshheading:14577578-Cloning, Molecular, pubmed-meshheading:14577578-Cricetinae, pubmed-meshheading:14577578-DNA, Complementary, pubmed-meshheading:14577578-DNA, Mitochondrial, pubmed-meshheading:14577578-DNA Primers, pubmed-meshheading:14577578-Electron Transport Complex IV, pubmed-meshheading:14577578-In Situ Hybridization, pubmed-meshheading:14577578-Mitochondria, Heart, pubmed-meshheading:14577578-Molecular Sequence Data, pubmed-meshheading:14577578-Mutation, Missense, pubmed-meshheading:14577578-Sequence Homology, Amino Acid, pubmed-meshheading:14577578-Sequence Homology, Nucleic Acid
pubmed:year
2003
pubmed:articleTitle
Identification of a new missense mutation in the mtDNA of hereditary hypertrophic, but not dilated cardiomyopathic hamsters.
pubmed:affiliation
Laboratorio di Cardiologia Molecolare e Cellulare, Dipartimento di Medicina Interna, Università di Roma 'Tor Vergata', Roma, Italy.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't