Linked Life Data

14576084

Source:http://linkedlifedata.com/resource/pubmed/id/14576084

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2004-1-6
pubmed:abstractText
Cytoskeletal reorganization of the smooth muscle cell in response to contractile stimulation may be an important fundamental process in regulation of tension development. We used confocal microscopy to analyze the effects of cholinergic stimulation on localization of the cytoskeletal proteins vinculin, paxillin, talin and focal adhesion kinase (FAK) in freshly dissociated tracheal smooth muscle cells. All four proteins were localized at the membrane and throughout the cytoplasm of unstimulated cells, but their concentration at the membrane was greater in acetylcholine (ACh)-stimulated cells. Antisense oligonucleotides were introduced into tracheal smooth muscle tissues to deplete paxillin protein, which also inhibited contraction in response to ACh. In cells dissociated from paxillin-depleted muscle tissues, redistribution of vinculin to the membrane in response to ACh was prevented, but redistribution of FAK and talin was not inhibited. Muscle tissues were transfected with plasmids encoding a paxillin mutant containing a deletion of the LIM3 domain (paxillin LIM3 dl 444-494), the primary determinant for targeting paxillin to focal adhesions. Expression of paxillin LIM3 dl in muscle tissues also inhibited contractile force and prevented cellular redistribution of paxillin and vinculin to the membrane in response to ACh, but paxillin LIM3 dl did not inhibit increases in intracellular Ca2+ or myosin light chain phosphorylation. Our results demonstrate that recruitment of paxillin and vinculin to smooth muscle membrane is necessary for tension development and that recruitment of vinculin to the membrane is regulated by paxillin. Vinculin and paxillin may participate in regulating the formation of linkages between the cytoskeleton and integrin proteins that mediate tension transmission between the contractile apparatus and the extracellular matrix during smooth muscle contraction.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0363-6143
pubmed:author
pubmed:issnType
Print
pubmed:volume
286
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
C433-47
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:14576084-Acetylcholine, pubmed-meshheading:14576084-Animals, pubmed-meshheading:14576084-Calcium, pubmed-meshheading:14576084-Cell Membrane, pubmed-meshheading:14576084-Cytoskeletal Proteins, pubmed-meshheading:14576084-Dogs, pubmed-meshheading:14576084-Focal Adhesion Protein-Tyrosine Kinases, pubmed-meshheading:14576084-Gene Deletion, pubmed-meshheading:14576084-Intracellular Membranes, pubmed-meshheading:14576084-Muscle, Smooth, pubmed-meshheading:14576084-Muscle Contraction, pubmed-meshheading:14576084-Mutation, pubmed-meshheading:14576084-Myosin Light Chains, pubmed-meshheading:14576084-Paxillin, pubmed-meshheading:14576084-Phosphoproteins, pubmed-meshheading:14576084-Phosphorylation, pubmed-meshheading:14576084-Protein Structure, Tertiary, pubmed-meshheading:14576084-Protein-Tyrosine Kinases, pubmed-meshheading:14576084-Talin, pubmed-meshheading:14576084-Tissue Distribution, pubmed-meshheading:14576084-Trachea, pubmed-meshheading:14576084-Vinculin
pubmed:year
2004
pubmed:articleTitle
Tension development during contractile stimulation of smooth muscle requires recruitment of paxillin and vinculin to the membrane.
pubmed:affiliation
Department of Cellular and Integrative Physiology, Indiana University School of Medicine, 635 Barnhill Dr., Indianapolis, IN 46202, USA.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't