Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3 Suppl
pubmed:dateCreated
2003-10-24
pubmed:abstractText
Nucleic acid sequence-based amplification (NASBA) allows the rapid amplification of specific regions of nucleic acid obtained from a diverse range of sources. It is especially suitable for amplifying RNA sequences. A NASBA technique was developed that allows the detection of avian influenza A subtype H5 from allantoic fluid harvested from inoculated chick embryos. The amplified viral RNA is detected by electrochemiluminescence. The described NASBA technique is a specific, rapid, and sensitive method of detection of influenza A subtype H5 viruses. More importantly, it can be used to distinguish high- and low-pathogenicity strains of the H5 subtype.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0005-2086
pubmed:author
pubmed:issnType
Print
pubmed:volume
47
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1069-74
pubmed:dateRevised
2006-3-30
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
A NASBA method to detect high- and low-pathogenicity H5 avian influenza viruses.
pubmed:affiliation
Hong Kong DNA Chips Ltd., 1805-6, 18F, Lu Plaza, 2 Wing Yip Street, Kowloon, Hong Kong SAR, China.
pubmed:publicationType
Journal Article