Source:http://linkedlifedata.com/resource/pubmed/id/14575113
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3 Suppl
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pubmed:dateCreated |
2003-10-24
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pubmed:abstractText |
Nucleic acid sequence-based amplification (NASBA) allows the rapid amplification of specific regions of nucleic acid obtained from a diverse range of sources. It is especially suitable for amplifying RNA sequences. A NASBA technique was developed that allows the detection of avian influenza A subtype H5 from allantoic fluid harvested from inoculated chick embryos. The amplified viral RNA is detected by electrochemiluminescence. The described NASBA technique is a specific, rapid, and sensitive method of detection of influenza A subtype H5 viruses. More importantly, it can be used to distinguish high- and low-pathogenicity strains of the H5 subtype.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
0005-2086
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
47
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1069-74
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pubmed:dateRevised |
2006-3-30
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pubmed:meshHeading |
pubmed-meshheading:14575113-Allantois,
pubmed-meshheading:14575113-Animals,
pubmed-meshheading:14575113-Base Sequence,
pubmed-meshheading:14575113-Birds,
pubmed-meshheading:14575113-Chick Embryo,
pubmed-meshheading:14575113-DNA Primers,
pubmed-meshheading:14575113-Influenza A virus,
pubmed-meshheading:14575113-Influenza in Birds,
pubmed-meshheading:14575113-RNA, Viral,
pubmed-meshheading:14575113-Self-Sustained Sequence Replication,
pubmed-meshheading:14575113-Sensitivity and Specificity
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pubmed:year |
2003
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pubmed:articleTitle |
A NASBA method to detect high- and low-pathogenicity H5 avian influenza viruses.
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pubmed:affiliation |
Hong Kong DNA Chips Ltd., 1805-6, 18F, Lu Plaza, 2 Wing Yip Street, Kowloon, Hong Kong SAR, China.
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pubmed:publicationType |
Journal Article
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