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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2003-12-17
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pubmed:abstractText |
Functional inducible NOS (iNOS) may be involved in the prolonged lifespan of chronic lymphocytic leukemia cells (B-CLL), although the exact mechanisms implicated remain elusive as yet. In this work, we have examined iNOS expression in normal B lymphocytes and B-CLL cells in pro- and antiapoptotic conditions. Our results demonstrate: (1) The existence of a new splice variant characterized by a complete deletion of exon 14 (iNOS 13-16(14del)), which was preferentially detected in normal B lymphocytes and may represent an isoform that could play a role in the regulation of enzyme activity. (2) The existence of another alternatively spliced iNOS mRNA transcript involving a partial deletion of the flavodoxin region (iNOS 13-16(neg)) was correlated to a decreased B-CLL cell viability. The 9-beta-D-arabinofuranosyl-2-fluoradenine or fludarabine (F-ara) treatment induced iNOS 13-16(neg) transcript variants, whereas IL-4 enhanced both the transcription of variants, including these exons (iNOS 13-16(pos)), and the expression of a 122 kDa iNOS protein. These results suggest that in B-CLL, a regulation process involving nitric oxide (.- NO) levels could occur by a post-transcriptional mechanism mediated by soluble factors. Our results also provide an insight into a new complementary proapoptotic action of F-ara in B-CLL by the induction of particular iNOS splice variants, leading to the activation of a caspase-3-dependent apoptotic pathway.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antineoplastic Agents,
http://linkedlifedata.com/resource/pubmed/chemical/CASP3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Caspase 3,
http://linkedlifedata.com/resource/pubmed/chemical/Caspases,
http://linkedlifedata.com/resource/pubmed/chemical/DNA (Cytosine-5-)-Methyltransferase,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4,
http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/NOS2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase Type II,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Vidarabine,
http://linkedlifedata.com/resource/pubmed/chemical/fludarabine
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0887-6924
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
18
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
48-56
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pubmed:dateRevised |
2011-10-27
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pubmed:meshHeading |
pubmed-meshheading:14574328-Aged,
pubmed-meshheading:14574328-Aged, 80 and over,
pubmed-meshheading:14574328-Alternative Splicing,
pubmed-meshheading:14574328-Antineoplastic Agents,
pubmed-meshheading:14574328-Apoptosis,
pubmed-meshheading:14574328-B-Lymphocytes,
pubmed-meshheading:14574328-Base Sequence,
pubmed-meshheading:14574328-Caspase 3,
pubmed-meshheading:14574328-Caspases,
pubmed-meshheading:14574328-DNA (Cytosine-5-)-Methyltransferase,
pubmed-meshheading:14574328-Female,
pubmed-meshheading:14574328-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:14574328-Gene Expression Regulation, Leukemic,
pubmed-meshheading:14574328-Humans,
pubmed-meshheading:14574328-Interleukin-4,
pubmed-meshheading:14574328-Isoenzymes,
pubmed-meshheading:14574328-Leukemia, Lymphocytic, Chronic, B-Cell,
pubmed-meshheading:14574328-Male,
pubmed-meshheading:14574328-Middle Aged,
pubmed-meshheading:14574328-Molecular Sequence Data,
pubmed-meshheading:14574328-Nitric Oxide,
pubmed-meshheading:14574328-Nitric Oxide Synthase,
pubmed-meshheading:14574328-Nitric Oxide Synthase Type II,
pubmed-meshheading:14574328-RNA, Messenger,
pubmed-meshheading:14574328-RNA Processing, Post-Transcriptional,
pubmed-meshheading:14574328-Sequence Deletion,
pubmed-meshheading:14574328-Sequence Homology, Nucleic Acid,
pubmed-meshheading:14574328-Signal Transduction,
pubmed-meshheading:14574328-Transcription, Genetic,
pubmed-meshheading:14574328-Vidarabine
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pubmed:year |
2004
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pubmed:articleTitle |
Post-transcriptional regulation of inducible nitric oxide synthase in chronic lymphocytic leukemia B cells in pro- and antiapoptotic culture conditions.
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pubmed:affiliation |
Departamento de Bioquímica, de la Facultad de Medicina, Universidad de la República, Montevideo, Uruguay.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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