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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
29
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pubmed:dateCreated |
2003-10-23
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pubmed:abstractText |
In developing neural tube, the basic helix-loop-helix (bHLH) transcription factor Olig2 interacts with the homeodomain transcription factor Nkx2.2 at two distinct stages. During neuronogenesis, a cross-repressive interaction appears to establish the precise boundary between the p3 and pMN domains. At later times, a cooperative interaction is noted because Nkx2.2 promotes maturation of oligodendrocyte progenitor cells specified by expression of Olig2. We show here that the Olig2 protein can form a physical complex with Nkx2.2 protein in mammalian cells and yeast two-hybrid trap assay. This interaction is specific because Olig2 does not bind to a biologically irrelevant homeodomain transcription factor (Nkx6.1), and Nkx2.2 does not interact with a biologically irrelevant bHLH protein (NeuroD). Deletion mapping analysis suggests that formation of an Olig2-Nkx2.2 physical complex is insufficient for the induction of oligodendrocyte progenitors in developing spine; however, the protein-protein interaction observed might be important for the cross-repressive interaction between Olig2 and Nkx2.2 that helps to establish the pMN-p3 boundary in the developing spinal cord.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1529-2401
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
22
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pubmed:volume |
23
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
9547-56
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:14573534-Animals,
pubmed-meshheading:14573534-Basic Helix-Loop-Helix Transcription Factors,
pubmed-meshheading:14573534-COS Cells,
pubmed-meshheading:14573534-Cell Nucleus,
pubmed-meshheading:14573534-Cells, Cultured,
pubmed-meshheading:14573534-Chick Embryo,
pubmed-meshheading:14573534-Homeodomain Proteins,
pubmed-meshheading:14573534-Macromolecular Substances,
pubmed-meshheading:14573534-Mice,
pubmed-meshheading:14573534-Nerve Tissue Proteins,
pubmed-meshheading:14573534-Protein Binding,
pubmed-meshheading:14573534-Protein Structure, Tertiary,
pubmed-meshheading:14573534-Recombinant Fusion Proteins,
pubmed-meshheading:14573534-Structure-Activity Relationship,
pubmed-meshheading:14573534-Transcription Factors,
pubmed-meshheading:14573534-Transfection,
pubmed-meshheading:14573534-Two-Hybrid System Techniques
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pubmed:year |
2003
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pubmed:articleTitle |
Cross-repressive interaction of the Olig2 and Nkx2.2 transcription factors in developing neural tube associated with formation of a specific physical complex.
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pubmed:affiliation |
Department of Pediatric Oncology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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