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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1978-2-18
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pubmed:abstractText |
Using a recently developed method of culturing T-lymphocyte colonies in agar-containing capillary tubes, the capacity of three different lymphoid extracts with lymphocyte chalone (LC) activity to inhibit colony growth was demonstrated. Sephadex fractions from a calf spleen extract were tested on the colony growth of granulocytic cells and PHA-stimulated T-lymphocytes as well as on the in vitro uptake of 3H-thymidine by bone marrow and ConA- and LPS-stimulated mouse spleen cells. The data strongly suggest that it is only the combination of several different assay systems applied to the same fractions that permits a clear-cut determination of a specific lymphocyte proliferation inhibitor like LC.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0340-6075
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
21
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pubmed:volume |
25
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
345-53
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:145728-Agar,
pubmed-meshheading:145728-Animals,
pubmed-meshheading:145728-Biological Assay,
pubmed-meshheading:145728-Cattle,
pubmed-meshheading:145728-Cell Division,
pubmed-meshheading:145728-Concanavalin A,
pubmed-meshheading:145728-Culture Techniques,
pubmed-meshheading:145728-Granulocytes,
pubmed-meshheading:145728-Growth Inhibitors,
pubmed-meshheading:145728-Lectins,
pubmed-meshheading:145728-Lipopolysaccharides,
pubmed-meshheading:145728-Lymph Nodes,
pubmed-meshheading:145728-Lymphocyte Activation,
pubmed-meshheading:145728-Lymphocytes,
pubmed-meshheading:145728-Mice,
pubmed-meshheading:145728-Spleen,
pubmed-meshheading:145728-T-Lymphocytes,
pubmed-meshheading:145728-Thymidine,
pubmed-meshheading:145728-Tissue Extracts
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pubmed:year |
1977
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pubmed:articleTitle |
A lymphocyte chalone assay using lymphocyte colony growth in agar capillaries.
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pubmed:publicationType |
Journal Article
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