Source:http://linkedlifedata.com/resource/pubmed/id/14569152
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
10
|
| pubmed:dateCreated |
2003-10-21
|
| pubmed:databankReference | |
| pubmed:abstractText |
A cDNA clone encoding the soluble guanylyl cyclase alpha2 subunit was isolated from medaka fish (Oryzias latipes) and designated as OlGCS-alpha2. The OlGCS-alpha2 cDNA was 3,192 bp in length and the open reading frame (ORF) encodes a protein of 805 amino acids. The deduced amino acid sequence has high similarity to that of the mammalian alpha2 subunit gene except for the N-terminal regulatory domain. The C-terminal 5 amino acids, "RETSL", which have been reported to interact with the post synaptic density protein (PSD)-95 were conserved. An RNase protection assay with adult fish organs showed that OlGCS-alpha2 was expressed mainly in the brain and testis. The complete nucleotide sequence (about 41 kbp) of the OlGCS-alpha2 genomic DNA clone isolated from a medaka fish BAC library indicated that the OlGCS-alpha2 gene consisted of 9 exons and 8 introns. The 5'-flanking region and larger introns, such as introns 1, 4, and 7, contained the several fragments conserved in the nucleotide sequences of Rex6 (non-long terminal repeat retrotransposon), MHC class I genomic region, and OlGC1, the medaka fish homolog of the mammalian guanylyl cyclase B gene. Linkage analysis on the medaka fish chromosome demonstrated that the OlGCS-alpha2 gene was mapped to LG13; this mapping position was different from those for the OlGCS-alpha1 and OlGCS-beta1 genes (LG1).
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0289-0003
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| pubmed:author |
pubmed-author:AsakawaShuichiS,
pubmed-author:HoriHiroshiH,
pubmed-author:MatsudaMasaruM,
pubmed-author:MitaniHiroshiH,
pubmed-author:NaruseKiyoshiK,
pubmed-author:ShimaAkihiroA,
pubmed-author:ShimizuNobuyoshiN,
pubmed-author:SuzukiNorioN,
pubmed-author:TsutsumiMakikoM,
pubmed-author:YamamotoTakehiroT,
pubmed-author:YaoYukoY
|
| pubmed:issnType |
Print
|
| pubmed:volume |
20
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1293-304
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:14569152-Amino Acid Sequence,
pubmed-meshheading:14569152-Animals,
pubmed-meshheading:14569152-Blotting, Northern,
pubmed-meshheading:14569152-Blotting, Southern,
pubmed-meshheading:14569152-Chromosome Mapping,
pubmed-meshheading:14569152-Chromosomes, Artificial, Bacterial,
pubmed-meshheading:14569152-DNA, Complementary,
pubmed-meshheading:14569152-DNA Primers,
pubmed-meshheading:14569152-Gene Expression Profiling,
pubmed-meshheading:14569152-Guanylate Cyclase,
pubmed-meshheading:14569152-Molecular Sequence Data,
pubmed-meshheading:14569152-Oryzias,
pubmed-meshheading:14569152-Phylogeny,
pubmed-meshheading:14569152-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:14569152-Ribonucleases,
pubmed-meshheading:14569152-Sequence Analysis, DNA
|
| pubmed:year |
2003
|
| pubmed:articleTitle |
Genomic structure and expression of the soluble guanylyl cyclase alpha2 subunit gene in the medaka fish Oryzias latipes.
|
| pubmed:affiliation |
Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo, Japan.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|