Source:http://linkedlifedata.com/resource/pubmed/id/14568682
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
2003-10-21
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| pubmed:abstractText |
Cytokeratin 8 (CK8) is one of the cytoskeletal components and shows caspase-mediated degradation when cells undergo apoptosis. We previously reported that CK8 is highly expressed in non-small cell lung cancer (NSCLC) cell lines and increasing values of serum CK8 are significantly associated with tumor progression in patients with NSCLC. In this investigation, reverse transcriptase-polymerase chain reaction (RT-PCR) analysis in lung cancer cell lines, revealed a shorter PCR product, which differed from the wild-type product of CK8. The nucleotide sequence of the shorter PCR products and genomic DNA for CK8 demonstrated that the shorter product was an aberrantly spliced form of CK8 (AS-CK8) which lacked a caspases cleavage site within the linker lesion in exon 5. The putative protein products predicted by the mRNA of AS-CK8 were demonstrated by Western blotting with monoclonal antibodies for CK8. In addition, AS-CK8 mRNA and its protein products were highly expressed in NSCLC cell lines compared with small-cell lung cancer (SCLC) cell lines. Tissue samples obtained from NSCLC patients also expressed mRNA of AS-CK8. In conclusion, we identified aberrantly spliced CK8 (AS-CK8) which lacked a caspases cleavage site in lung cancer cell lines and primary tumors of NSCLC. AS-CK8 was preferentially expressed in NSCLC, rather than SCLC. These findings lead to speculation that cancer cells expressing AS-CK8 may have a resistance to apoptosis and may perturb keratin network formation.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0169-5002
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| pubmed:author |
pubmed-author:BandohShujiS,
pubmed-author:FujitaJiroJ,
pubmed-author:FukunagaYokoY,
pubmed-author:HuangCheng-LongCL,
pubmed-author:IshidaToshihikoT,
pubmed-author:IshiiTomoyaT,
pubmed-author:KuboAkihitoA,
pubmed-author:TojoYasunoriY,
pubmed-author:UedaYutakaY,
pubmed-author:WuFeiF,
pubmed-author:YangYuY,
pubmed-author:YokomiseHiroyasuH
|
| pubmed:issnType |
Print
|
| pubmed:volume |
42
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
153-61
|
| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:14568682-Apoptosis,
pubmed-meshheading:14568682-Base Sequence,
pubmed-meshheading:14568682-Carcinoma, Non-Small-Cell Lung,
pubmed-meshheading:14568682-Carcinoma, Small Cell,
pubmed-meshheading:14568682-Humans,
pubmed-meshheading:14568682-Keratins,
pubmed-meshheading:14568682-Lung Neoplasms,
pubmed-meshheading:14568682-Molecular Sequence Data,
pubmed-meshheading:14568682-RNA, Messenger,
pubmed-meshheading:14568682-RNA Splice Sites,
pubmed-meshheading:14568682-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:14568682-Tumor Cells, Cultured
|
| pubmed:year |
2003
|
| pubmed:articleTitle |
Aberrant messenger RNA splicing of the cytokeratin 8 in lung cancer.
|
| pubmed:affiliation |
First Department of Internal Medicine, Kagawa Medical University, 1750-1, Miki-cho, Kita-gun, Kagawa 761-0793, Japan.
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| pubmed:publicationType |
Journal Article
|