Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2003-10-21
pubmed:abstractText
A key step in RNA interference (RNAi) is assembly of the RISC, the protein-siRNA complex that mediates target RNA cleavage. Here, we show that the two strands of an siRNA duplex are not equally eligible for assembly into RISC. Rather, both the absolute and relative stabilities of the base pairs at the 5' ends of the two siRNA strands determine the degree to which each strand participates in the RNAi pathway. siRNA duplexes can be functionally asymmetric, with only one of the two strands able to trigger RNAi. Asymmetry is the hallmark of a related class of small, single-stranded, noncoding RNAs, microRNAs (miRNAs). We suggest that single-stranded miRNAs are initially generated as siRNA-like duplexes whose structures predestine one strand to enter the RISC and the other strand to be destroyed. Thus, the common step of RISC assembly is an unexpected source of asymmetry for both siRNA function and miRNA biogenesis.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Diphosphate, http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphate, http://linkedlifedata.com/resource/pubmed/chemical/MicroRNAs, http://linkedlifedata.com/resource/pubmed/chemical/RNA, Antisense, http://linkedlifedata.com/resource/pubmed/chemical/RNA, Double-Stranded, http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger, http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering, http://linkedlifedata.com/resource/pubmed/chemical/RNA, Untranslated, http://linkedlifedata.com/resource/pubmed/chemical/RNA Helicases, http://linkedlifedata.com/resource/pubmed/chemical/RNA-Induced Silencing Complex, http://linkedlifedata.com/resource/pubmed/chemical/Superoxide Dismutase, http://linkedlifedata.com/resource/pubmed/chemical/superoxide dismutase 1
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0092-8674
pubmed:author
pubmed:issnType
Print
pubmed:day
17
pubmed:volume
115
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
199-208
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:14567917-Adenosine Diphosphate, pubmed-meshheading:14567917-Adenosine Triphosphate, pubmed-meshheading:14567917-Animals, pubmed-meshheading:14567917-Base Pair Mismatch, pubmed-meshheading:14567917-Base Pairing, pubmed-meshheading:14567917-Base Sequence, pubmed-meshheading:14567917-Drosophila melanogaster, pubmed-meshheading:14567917-Hydrogen Bonding, pubmed-meshheading:14567917-Kinetics, pubmed-meshheading:14567917-MicroRNAs, pubmed-meshheading:14567917-Models, Biological, pubmed-meshheading:14567917-Phosphorylation, pubmed-meshheading:14567917-RNA, Antisense, pubmed-meshheading:14567917-RNA, Double-Stranded, pubmed-meshheading:14567917-RNA, Messenger, pubmed-meshheading:14567917-RNA, Small Interfering, pubmed-meshheading:14567917-RNA, Untranslated, pubmed-meshheading:14567917-RNA Helicases, pubmed-meshheading:14567917-RNA Interference, pubmed-meshheading:14567917-RNA-Induced Silencing Complex, pubmed-meshheading:14567917-Superoxide Dismutase
pubmed:year
2003
pubmed:articleTitle
Asymmetry in the assembly of the RNAi enzyme complex.
pubmed:affiliation
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Lazare Research Building, 364 Plantation Street, Worcester, MA 01605, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't