|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0030685,
umls-concept:C0040711,
umls-concept:C0391871,
umls-concept:C0441712,
umls-concept:C0680255,
umls-concept:C0851285,
umls-concept:C1283071,
umls-concept:C1422537,
umls-concept:C1622421,
umls-concept:C1963578,
umls-concept:C2587213
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
2003-10-21
|
|
pubmed:abstractText |
Transcript-specific translational control is generally directed by binding of trans-acting proteins to structural elements in the untranslated region (UTR) of the target mRNA. Here, we elucidate a translational silencing mechanism involving regulated release of an integral ribosomal protein and subsequent binding to its target mRNA. Human ribosomal protein L13a was identified as a candidate interferon-Gamma-Activated Inhibitor of Translation (GAIT) of ceruloplasmin (Cp) mRNA by a genetic screen for Cp 3'-UTR binding proteins. In vitro activity of L13a was shown by inhibition of target mRNA translation by recombinant protein. In response to interferon-gamma in vivo, the entire cellular pool of L13a was phosphorylated and released from the 60S ribosomal subunit. Released L13a specifically bound the 3'-UTR GAIT element of Cp mRNA and silenced translation. We propose a model in which the ribosome functions not only as a protein synthesis machine, but also as a depot for regulatory proteins that modulate translation.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Oct
|
|
pubmed:issn |
0092-8674
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
17
|
|
pubmed:volume |
115
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
187-98
|
|
pubmed:dateRevised |
2009-2-11
|
|
pubmed:meshHeading |
pubmed-meshheading:14567916-3' Untranslated Regions,
pubmed-meshheading:14567916-Amino Acid Sequence,
pubmed-meshheading:14567916-Ceruloplasmin,
pubmed-meshheading:14567916-Gene Expression Regulation,
pubmed-meshheading:14567916-Gene Silencing,
pubmed-meshheading:14567916-Humans,
pubmed-meshheading:14567916-Interferon-gamma,
pubmed-meshheading:14567916-Models, Biological,
pubmed-meshheading:14567916-Models, Molecular,
pubmed-meshheading:14567916-Molecular Sequence Data,
pubmed-meshheading:14567916-Molecular Structure,
pubmed-meshheading:14567916-Phosphorylation,
pubmed-meshheading:14567916-Protein Biosynthesis,
pubmed-meshheading:14567916-Protein Structure, Tertiary,
pubmed-meshheading:14567916-RNA, Messenger,
pubmed-meshheading:14567916-RNA Processing, Post-Transcriptional,
pubmed-meshheading:14567916-Recombinant Proteins,
pubmed-meshheading:14567916-Ribosomal Proteins,
pubmed-meshheading:14567916-Ribosomes,
pubmed-meshheading:14567916-Time Factors,
pubmed-meshheading:14567916-U937 Cells
|
|
pubmed:year |
2003
|
|
pubmed:articleTitle |
Regulated release of L13a from the 60S ribosomal subunit as a mechanism of transcript-specific translational control.
|
|
pubmed:affiliation |
Department of Cell Biology, Lerner Research Institute, Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|
