Source:http://linkedlifedata.com/resource/pubmed/id/14566671
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
2003-10-20
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| pubmed:abstractText |
Long-term renal allograft survival is limited mainly by the progressive process termed chronic allograft nephropathy (CAN) or chronic rejection. A pathological feature of CAN is characterized by progressive interstitial fibrosis. Transforming growth factor (TGF)-beta(1) plays an important role in fibrogenesis. We investigated whether the degree of TGF-beta(1) expression in early biopsy specimens routinely obtained from stable allografts at 100 days could predict fibrosis and graft dysfunction in the late phase by immunohistochemistry. Patients were children with a graft from related donors. We immunohistochemically determined intracellular and extracellular expression of TGF-beta(1) in the graft at 100 days using LC antibody (LC) for intracellular TGF-beta(1) and CC antibody (CC) for extracellular TGF-beta(1). We used the change in creatinine clearance between 100 days and 3 years after transplantation (Delta Ccr) as an index of long-term graft function. Image analysis was used to calculate the relative area involved by interstitial fibrosis in trichrome-stained sections of graft biopsy specimens at 100 days and 3 years, designating the change as Delta FI. Delta Ccr was - 4.2 +/- 9.4 mL/min in subjects with minimal early immunoreactivity for CC and - 20.5 +/- 5.9 mL/min in subjects with strong reactivity (p < 0.05). Delta Ccr was - 14.5 +/- 18.6 mL/min in subjects with minimal early immunoreactivity for LC and - 11.7 +/- 12.8 mL/min in those with strong reactivity. Delta FI in subjects with minimal CC reactivity (1.28 +/- 4.11 %) tended to be lower than in subjects with strong reactivity (8.45 +/- 15.47 %). Neither fibrosis at 100 days nor Delta FI differed between subjects with minimal and strong LC reactivity. Thus, extracellular TGF-beta(1) expression in grafts at 100 days after transplantation has an influence on long-term graft function and tends to be associated with increased graft fibrosis at 3 years.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Jul
|
| pubmed:issn |
0001-7868
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
34
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
234-8
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:14566671-Adolescent,
pubmed-meshheading:14566671-Age Factors,
pubmed-meshheading:14566671-Biopsy,
pubmed-meshheading:14566671-Child,
pubmed-meshheading:14566671-Chronic Disease,
pubmed-meshheading:14566671-Data Interpretation, Statistical,
pubmed-meshheading:14566671-Female,
pubmed-meshheading:14566671-Fibrosis,
pubmed-meshheading:14566671-Graft Rejection,
pubmed-meshheading:14566671-Humans,
pubmed-meshheading:14566671-Immunohistochemistry,
pubmed-meshheading:14566671-Kidney,
pubmed-meshheading:14566671-Kidney Diseases,
pubmed-meshheading:14566671-Kidney Transplantation,
pubmed-meshheading:14566671-Male,
pubmed-meshheading:14566671-Prognosis,
pubmed-meshheading:14566671-Risk Factors,
pubmed-meshheading:14566671-Staining and Labeling,
pubmed-meshheading:14566671-Time Factors,
pubmed-meshheading:14566671-Transforming Growth Factor beta,
pubmed-meshheading:14566671-Transplantation, Homologous
|
| pubmed:year |
2003
|
| pubmed:articleTitle |
Significance of early biopsy in pediatric kidney transplantation.
|
| pubmed:affiliation |
Department of Urology, Kawasaki Medical School, Matsushima, Kurashika, Japan.
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| pubmed:publicationType |
Journal Article,
Comparative Study
|