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rdf:type |
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lifeskim:mentions |
umls-concept:C0003018,
umls-concept:C0006675,
umls-concept:C0007634,
umls-concept:C0040649,
umls-concept:C0086418,
umls-concept:C0169661,
umls-concept:C0290758,
umls-concept:C1514873,
umls-concept:C1546857,
umls-concept:C1556066,
umls-concept:C1619636,
umls-concept:C1879547
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pubmed:issue |
52
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pubmed:dateCreated |
2003-12-22
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pubmed:abstractText |
Angiotensin II- and K+-stimulated aldosterone production in the adrenocortical glomerulosa cells requires induction of the steroidogenic acute regulatory protein (StAR). While both agents activate Ca2+ signaling, the mechanisms leading to aldosterone synthesis are distinct, and the angiotensin II response cannot be mimicked by K+. We previously reported that StAR mRNA levels and promoter-reporter gene activity in transiently transfected H295R human adrenocortical cells were stimulated by angiotensin II but not by K+ treatment. The current study focused on identifying signaling pathways activated by angiotensin II that contribute to StAR transcriptional activation. We show that the angiotensin II-stimulated transcriptional activation of StAR was dependent upon influx of external calcium and requires protein kinase C activation. Furthermore we describe for the first time that the Janus tyrosine kinase family member, JAK2, was activated by angiotensin II treatment of H295R cells. Treatment of the cells with AG490, a selective inhibitor of JAK2, blocked JAK2 activation and StAR reporter gene activity and inhibited steroid production. Taken together these studies describe a novel pathway controlling StAR expression and steroidogenesis in adrenocortical cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Angiotensin II,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media, Serum-Free,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Flavonoids,
http://linkedlifedata.com/resource/pubmed/chemical/JAK2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Janus Kinase 2,
http://linkedlifedata.com/resource/pubmed/chemical/Luciferases,
http://linkedlifedata.com/resource/pubmed/chemical/PD 98059,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Progesterone,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Tyrphostins,
http://linkedlifedata.com/resource/pubmed/chemical/alpha-cyano-(3,4-dihydroxy)-N-benzyl...,
http://linkedlifedata.com/resource/pubmed/chemical/steroidogenic acute regulatory...
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
26
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pubmed:volume |
278
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
52355-62
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:14565954-Adrenal Cortex,
pubmed-meshheading:14565954-Angiotensin II,
pubmed-meshheading:14565954-Calcium,
pubmed-meshheading:14565954-Cell Line,
pubmed-meshheading:14565954-Culture Media, Serum-Free,
pubmed-meshheading:14565954-Dose-Response Relationship, Drug,
pubmed-meshheading:14565954-Enzyme Inhibitors,
pubmed-meshheading:14565954-Flavonoids,
pubmed-meshheading:14565954-Genes, Reporter,
pubmed-meshheading:14565954-Humans,
pubmed-meshheading:14565954-Immunoblotting,
pubmed-meshheading:14565954-Janus Kinase 2,
pubmed-meshheading:14565954-Luciferases,
pubmed-meshheading:14565954-Phosphoproteins,
pubmed-meshheading:14565954-Progesterone,
pubmed-meshheading:14565954-Promoter Regions, Genetic,
pubmed-meshheading:14565954-Protein-Tyrosine Kinases,
pubmed-meshheading:14565954-Proto-Oncogene Proteins,
pubmed-meshheading:14565954-RNA, Messenger,
pubmed-meshheading:14565954-Radioimmunoassay,
pubmed-meshheading:14565954-Signal Transduction,
pubmed-meshheading:14565954-Time Factors,
pubmed-meshheading:14565954-Transcription, Genetic,
pubmed-meshheading:14565954-Transcriptional Activation,
pubmed-meshheading:14565954-Transfection,
pubmed-meshheading:14565954-Tyrphostins
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pubmed:year |
2003
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pubmed:articleTitle |
Janus kinase 2 and calcium are required for angiotensin II-dependent activation of steroidogenic acute regulatory protein transcription in H295R human adrenocortical cells.
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pubmed:affiliation |
Department of Biochemistry and Molecular Biology, School of Medicine, University of Louisville, Louisville, Kentucky 40292, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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