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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1993-1-7
|
| pubmed:abstractText |
We previously described a novel 68,000 D macrophage-derived protein (MMS-68) that can stimulate mucus-like glycoconjugate (MLGC) secretion from cultured human airways, respiratory epithelial cells, and the ishikawa adenocarcinoma cell line. To better characterize this mucus secretagogue, we generated monoclonal antibodies against MMS-68 by injecting crushed SDS-PAGE gel slices containing this protein into Balb-C mice followed by fusion with SP2/0, a nonsecreting mouse myeloma cell line. A panel of monoclonal antibodies was produced that identified the 68,000 D MMS by immunoblot analysis and immunoprecipitation. The monoclonal antibodies detected MMS-68 in normal peripheral blood monocytes and pulmonary macrophages by cytofluorographic analysis and in human airways as determined by immunohistochemistry. Utilizing the monoclonal antibodies, an antigen-capture ELISA assay was developed. Statistically significant elevations in levels of MMS-68 were detected in bronchoalveolar lavage fluid (BALF) of chronic bronchitic subjects and cigarette smokers and in monocyte culture supernatants from steroid-dependent asthmatic patients compared to normal control subjects. The 68,000 D MMS is a potent secretagogue and may play an important role in the regulation of mucus secretion, especially in chronic bronchitis and steroid-dependent asthma.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0003-0805
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
146
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1589-97
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:1456580-Adult,
pubmed-meshheading:1456580-Animals,
pubmed-meshheading:1456580-Antibodies, Monoclonal,
pubmed-meshheading:1456580-Asthma,
pubmed-meshheading:1456580-Bronchitis,
pubmed-meshheading:1456580-Bronchoalveolar Lavage Fluid,
pubmed-meshheading:1456580-Cells, Cultured,
pubmed-meshheading:1456580-Chromatography, Affinity,
pubmed-meshheading:1456580-Cytoplasm,
pubmed-meshheading:1456580-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:1456580-Female,
pubmed-meshheading:1456580-Fluorescent Antibody Technique,
pubmed-meshheading:1456580-Glycoconjugates,
pubmed-meshheading:1456580-Guinea Pigs,
pubmed-meshheading:1456580-Humans,
pubmed-meshheading:1456580-Immunoblotting,
pubmed-meshheading:1456580-Immunohistochemistry,
pubmed-meshheading:1456580-Infection,
pubmed-meshheading:1456580-Macrophages, Alveolar,
pubmed-meshheading:1456580-Male,
pubmed-meshheading:1456580-Mice,
pubmed-meshheading:1456580-Mice, Inbred BALB C,
pubmed-meshheading:1456580-Middle Aged,
pubmed-meshheading:1456580-Monocytes,
pubmed-meshheading:1456580-Mucus,
pubmed-meshheading:1456580-Precipitin Tests,
pubmed-meshheading:1456580-Smoking
|
| pubmed:year |
1992
|
| pubmed:articleTitle |
In vivo detection of a novel macrophage-derived protein involved in the regulation of mucus-like glycoconjugate secretion.
|
| pubmed:affiliation |
Department of Medicine, Mount Sinai Medical Center, New York, New York.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|