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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
46
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pubmed:dateCreated |
2003-10-16
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pubmed:abstractText |
T lymphocytes infiltrating a human lung carcinoma stimulated in vitro with autologous tumor cell line showed a TCRVbeta13.6(+) T-cell expansion. This subset was isolated using TCRVbeta-specific antibody and several T-cell clones were generated. All these clones expressed a unique Vbeta13.6-Jbeta2.7 TCR with the same junctional region strongly suggesting that they derived from the same cell. They were CD8(+)/CD28(-) and expressed the MHC class I binding killer cell Ig-like receptor (KIR)3DL2/p140, but not KIR3DL1/p70, KIR2DL1/p58.1 and KIR2DL2/3/p58.2. Sequence analysis indicated that KIR3DL2/p140 cDNA was identical to the previously reported 3DL2*002 allele except for two nucleic acid substitutions. Functional studies showed that KIR3DL2/p140(+) CTL secrete a significant level of IFNgamma and mediate an HLA-A2-restricted cytotoxicity against the autologous and some allogeneic tumor cells but not towards the autologous EBV-B cells. Strikingly, both the lytic and the cytokine secretion activities induced upon specific cell interactions were unaffected by anti-KIR3DL2/p140 antibody. In addition, crosslinking KIR3DL2/p140 molecules on CTL did not result into the modification of cytotoxicity and cytokine production triggered by anti-CD3 antibody. These results strongly suggest that, as opposed to distinct KIR expressed by CTL, the in vitro KIR3DL2/p140 engagement does not result into inhibitory (nor activatory) effects on tumor-specific CTL.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/KIR2DL2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/KIR2DL3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/KIR3DL1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/KIR3DL2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, KIR,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, KIR2DL1,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, KIR2DL2,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, KIR2DL3,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, KIR3DL1,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, KIR3DL2
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0950-9232
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
16
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pubmed:volume |
22
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7192-8
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:14562047-Antigens, CD,
pubmed-meshheading:14562047-Carcinoma, Non-Small-Cell Lung,
pubmed-meshheading:14562047-Clone Cells,
pubmed-meshheading:14562047-Cytotoxicity, Immunologic,
pubmed-meshheading:14562047-Fluorescent Antibody Technique,
pubmed-meshheading:14562047-Humans,
pubmed-meshheading:14562047-Killer Cells, Natural,
pubmed-meshheading:14562047-Lung Neoplasms,
pubmed-meshheading:14562047-Lymphocytes, Tumor-Infiltrating,
pubmed-meshheading:14562047-Receptors, Antigen, T-Cell, alpha-beta,
pubmed-meshheading:14562047-Receptors, Immunologic,
pubmed-meshheading:14562047-Receptors, KIR,
pubmed-meshheading:14562047-Receptors, KIR2DL1,
pubmed-meshheading:14562047-Receptors, KIR2DL2,
pubmed-meshheading:14562047-Receptors, KIR2DL3,
pubmed-meshheading:14562047-Receptors, KIR3DL1,
pubmed-meshheading:14562047-Receptors, KIR3DL2,
pubmed-meshheading:14562047-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:14562047-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:14562047-Tumor Cells, Cultured
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pubmed:year |
2003
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pubmed:articleTitle |
Functional and molecular characterization of a KIR3DL2/p140 expressing tumor-specific cytotoxic T lymphocyte clone infiltrating a human lung carcinoma.
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pubmed:affiliation |
Laboratoire Cytokines et Immunologie des tumeurs Humaines, INSERM U487, Institut Gustave Roussy, F-94805 Villejuif, Cedex, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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