Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2004-1-26
pubmed:abstractText
It is still not fully understood to what extent intronic sequences contribute to the regulation of the different forms of alternative splicing. We are interested in the regulation of alternative cassette exon events, such as exon inclusion and exon skipping. We investigated these events by comparative genomic analysis of human and mouse in five experimentally well-characterized genes, neurofibromatosis 1 (NF1), cystic fibrosis transmembrane conductance regulator (CFTR), period 3 (PER3), cysteinyl-tRNA synthetase (CARS) and synaptotagmin 7 (SYT7). In NF1, high intron identity around the 52 constitutive and four alternatively skipped NF1 exons is restricted to the close vicinity of the exons. In contrast, we found on average high conservation of intron sequences over 300 base pairs up- and downstream of the five alternatively included NF1 exons. The investigation of alternatively included exons in CFTR, PER3, CARS and SYT7 supported this finding. In contrast, the mean intron identities around the alternatively skipped exons in CTFR and NF1 do not differ considerably from those around the constitutive exons. In these genes, the difference in intron conservation could point to a difference between the regulation of alternative exon inclusion and alternative exon skipping or constitutive exon splicing. Additional genome-wide investigations are necessary to elucidate to what extent our finding can be generalized.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Amino Acyl-tRNA Synthetases, http://linkedlifedata.com/resource/pubmed/chemical/CFTR protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Binding Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Cystic Fibrosis Transmembrane..., http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers, http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins, http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins, http://linkedlifedata.com/resource/pubmed/chemical/PER3 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Per3 protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Period Circadian Proteins, http://linkedlifedata.com/resource/pubmed/chemical/SYT7 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Synaptotagmins, http://linkedlifedata.com/resource/pubmed/chemical/Syt7 protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors, http://linkedlifedata.com/resource/pubmed/chemical/cysteinyl-tRNA synthetase
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1018-4813
pubmed:author
pubmed:issnType
Print
pubmed:volume
12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
139-49
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:14560314-Alternative Splicing, pubmed-meshheading:14560314-Amino Acyl-tRNA Synthetases, pubmed-meshheading:14560314-Animals, pubmed-meshheading:14560314-Base Sequence, pubmed-meshheading:14560314-Calcium-Binding Proteins, pubmed-meshheading:14560314-Cystic Fibrosis Transmembrane Conductance Regulator, pubmed-meshheading:14560314-DNA Primers, pubmed-meshheading:14560314-Exons, pubmed-meshheading:14560314-Genes, Neurofibromatosis 1, pubmed-meshheading:14560314-Humans, pubmed-meshheading:14560314-Introns, pubmed-meshheading:14560314-Membrane Glycoproteins, pubmed-meshheading:14560314-Mice, pubmed-meshheading:14560314-Nerve Tissue Proteins, pubmed-meshheading:14560314-Nuclear Proteins, pubmed-meshheading:14560314-Period Circadian Proteins, pubmed-meshheading:14560314-Sequence Homology, Nucleic Acid, pubmed-meshheading:14560314-Synaptotagmins, pubmed-meshheading:14560314-Transcription Factors
pubmed:year
2004
pubmed:articleTitle
In NF1, CFTR, PER3, CARS and SYT7, alternatively included exons show higher conservation of surrounding intron sequences than constitutive exons.
pubmed:affiliation
Department of Human Genetics, University of Ulm, Germany. dieter.kaufmann@medizin.uni-ulm.de
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't