Linked Life Data

14559902

Source:http://linkedlifedata.com/resource/pubmed/id/14559902

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
52
pubmed:dateCreated
2003-12-22
pubmed:abstractText
Phospholipid lipid transfer protein (PLTP) is ubiquitously expressed in animal tissues and plays multiple roles in lipoprotein metabolism, but the function of peripheral PLTP is still poorly understood. Here we show that one of its possible functions is to transport cholesterol and phospholipids from cells to lipoprotein particles by a process involving PLTP interactions with cellular ATP-binding cassette transporter A1 (ABCA1). When ABCA1 was induced in murine macrophages or ABCA1-transfected baby hamster kidney cells, PLTP gained the ability to promote cholesterol and phospholipid efflux from cells. Although PLTP alone had lipid efflux activity, its maximum activity was observed in the presence of high density lipoprotein particles. Pulsechase studies showed that the interaction of PLTP with ABCA1-expressing cells played a role in promoting lipid efflux. Overexpression of ABCA1 dramatically increased binding of both PLTP and apoA-I to common sites on the cell surface. Both PLTP and apoA-I were covalently cross-linked to ABCA1, each protein blocked cross-linking of the other, and both PLTP and apoA-I stabilized ABCA1 protein. These results are consistent with PLTP and apoA-I binding to ABCA1 at the same or closely related sites. Thus, PLTP mimics apolipoproteins in removing cellular lipids by the ABCA1 pathway, except that PLTP acts more as an intermediary in the transfer of cellular lipids to lipoprotein particles.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
26
pubmed:volume
278
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
52379-85
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:14559902-ATP-Binding Cassette Transporters, pubmed-meshheading:14559902-Animals, pubmed-meshheading:14559902-Carrier Proteins, pubmed-meshheading:14559902-Cell Line, pubmed-meshheading:14559902-Cholesterol, pubmed-meshheading:14559902-Cricetinae, pubmed-meshheading:14559902-DNA, Complementary, pubmed-meshheading:14559902-Dose-Response Relationship, Drug, pubmed-meshheading:14559902-Fibroblasts, pubmed-meshheading:14559902-Humans, pubmed-meshheading:14559902-Lipid Metabolism, pubmed-meshheading:14559902-Lipoproteins, pubmed-meshheading:14559902-Macrophages, pubmed-meshheading:14559902-Membrane Proteins, pubmed-meshheading:14559902-Mice, pubmed-meshheading:14559902-Mutation, pubmed-meshheading:14559902-Phospholipid Transfer Proteins, pubmed-meshheading:14559902-Phospholipids, pubmed-meshheading:14559902-Protein Binding, pubmed-meshheading:14559902-Recombinant Proteins, pubmed-meshheading:14559902-Time Factors, pubmed-meshheading:14559902-Transfection
pubmed:year
2003
pubmed:articleTitle
Phospholipid transfer protein interacts with and stabilizes ATP-binding cassette transporter A1 and enhances cholesterol efflux from cells.
pubmed:affiliation
Department of Medicine, University of Washington, Seattle, Washington 98195-6426, USA. joram@u.washington.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.